无水保活运输温度对史氏鲟氧化应激的影响

为了提高史氏鲟的保活时间和存活率，选取合适质量浓度MS-222麻醉史氏鲟，研究不同无水保活运输温度（4、8、12 ℃）对史氏鲟氧化应激的影响，分别记录模拟保活运输12 h和24 h后各温度组的存活率，分析保活运输前后应激指标和抗氧化指标变化。结果表明：MS-222麻醉史氏鲟，较佳镇静质量浓度为100 mg/L、镇静时间为（2.1±0.1） min，较佳麻醉质量浓度为110 mg/L、麻醉时间为（2.1±0.3） min；保活运输12 h时，12 ℃条件下存活率最高，为85%，保活运输24 h时，4 ℃条件下存活率最高，为62.5%；保活运输12 h后12 ℃条件下血糖浓度和皮质醇含量显著低于其他组，超氧化物歧化酶和谷胱甘肽过氧化物酶（glutathione peroxidase，GPX）活性显著低于其他组，同时丙二醛（malondialdehyde，MDA）含量最低（P＜0.05），表明受到的应激最小；保活运输24 h时，各温度组过氧化氢酶活性无明显差异，4 ℃条件下GPX活性较低，同时MDA含量显著低于其他组（P＜0.05）。因此在史氏鲟短时间（12 h）保活运输时，较高温度（12 ℃）减少应激效果明显，是较适的保活温度；在较长时间（24 h） 保活运输时，低温能更好保持低代谢和麻醉状态，4 ℃是较适的保活温度。

Effects of Different Temperatures during Waterless Live Transportation on Oxidative Stress in Amur Sturgeon (Acipenser schrencki)

In order to improve the survival time and survival rate of Amur sturgeon, the fish were anaesthetized with an appropriate concentration of MS-222 to explore the effects of different temperatures (4, 8 and 12 ℃) during waterless live transportation on oxidative stress in it. The survival rate of the fish was recorded after 12 and 24 h of simulated live transportation, and the changes in oxidative stress and antioxidant indicators were analyzed before and after live transportation. The results showed that the appropriate sedative concentration of MS-222 was 100 mg/L and the sedation time was (2.1 ± 0.1) min; the appropriate anaesthetic concentration was 110 mg/L and the anesthesia time was (2.1 ± 0.3) min. After 12 h live transportation, the highest survival rate of 85% was observed at 12 ℃. After 24 h live transportation, the highest survival rate of 62.5% was observed at 4 ℃. After 12 h live transportation, blood sugar, cortisol and malondialdehyde (MDA) levels as well as the activity of superoxide dismutase (SOD) and glutathione peroxidase (GPX) were significantly lower in the 12 ℃ group than in the other temperature groups (P < 0.05), indicating that the lowest level of oxidative stress occurred in the 12 ℃ group. After 24 h live transportation, there were no significant differences in catalase (CAT) activity among all groups. GPX activity was lower in the 4 ℃ group, and MDA content was significantly lower in this group than in the other groups (P < 0.05). Therefore, when the sturgeon was transported without water for a short time (12 h), a significantly lower level of oxidative stress was observed at higher temperature (12 ℃), suggesting that the appreciate survival temperature was 12 ℃. When the sturgeon was transported for a long time (24 h), low metabolism and anesthesia were maintained better at lower temperature (4 ℃), so that the fish could survive better.