Surface modification of silica glass by CHF3 plasma treatment and carbon negative-ion implantation for cell pattern adhesion |
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Authors: | Hiroshi Tsuji Piyanuch SommaniYuichiro Hayashi Hiroyuki KojimaHiroko Sato Yasuhito GotohGikan Takaoka Junzo Ishikawa |
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Affiliation: | a Department of Electronic Science and Engineering, Kyoto University, Japanb Photonics and Electronic Science and Engineering Center, Kyoto University, Japanc Department of Electronics and Information Engineering, Chubu University, Japan |
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Abstract: | We have investigated a method for the patterning of cell adhesion on a silica glass by using two-steps of surface modification processes of CHF3 plasma treatment and negative-ion pattern implantation. For the first step, exposure of CHF3 plasma to silica glass (SG) was used to obtain hydrophobic surface, leading to eliminate cell-adhesion property. After treatment with RF power of 20 W and exposure time of 120 s, the hydrophobicity was occurred from the increase in contact angle of SG from 43° to 88° and its reason based on XPS analysis was due to formations of C―F, C―F2, and C―F3 bonds, so-called fluorocarbonated bonds. Culture of mesenchymal stem cells (MSC) and rat adrenal pheochromocytoma cells (PC12h) showed the degradation of cell adhesion property on the plasma-treated SG surface. For the second step, carbon negative-ion implantation into the hydrophobic fluorocarbonated-SG surface was used to pattern the hydrophilic region, leading to enhance cell adhesion property. The contact angle of C-modified surface decreased to 76° at conditions of 15 keV and 1 × 1015 ions/cm2. XPS showed that the hydrophilicity was due to reduction of C―Fx bonds and formation of C―O and C═O bonds. After 3 days culture of MSC and PC12h on the C-implanted surface of the plasma-treated SG, a fairly good adhesion patterning of both cells was obtained on the ion-implanted regions. |
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Keywords: | Plasma treatment Ion implantation Cell adhesion Silica glass Contact angle Stem cell |
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