Isoflavonoid production by soy plant callus suspension culture |
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| Authors: | Alper Gueven Dietrich Knorr |
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| Affiliation: | a Tunceli University, Food Engineering Department, Tunceli 62100, Turkey
b Berlin University of Technology, Department of Food Biotech. and Food Process Eng., Koenigin-Luise-Str. 22, D-14195 Berlin, Germany |
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| Abstract: | Production of aglycone type isoflavonoids, Genistein and Daidzein, and β-glycosidic type isoflavonoids, Genistin and Daidzin, by the soy plant callus suspension culture was investigated in a batch system. Genistein and Daidzein concentrations increased during the exponential growth phase of the biomass. Genistin and Daidzin concentrations were not directly related with biomass production. Methyl jasmonate addition to the growth medium (0.1 kg/m3) was found to increase isoflavonoid biosynthesis by 5-6 times. Isoflavonoid production in the 7 days old culture increased with pulsed electric field (PEF) application at 1.6 kV, and aglycone forms were influenced to a greater extent. |
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| Keywords: | Plant tissue culture Isoflavonoids Genistein Daidzein Genistin Daidzin Elicitor Precursor Pulsed electric field High hydrostatic pressure |
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