| 膜分离在蛋白质分离纯化中的应用 |
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| 引用本文： | 姚红娟,王晓琳,丁宁. 膜分离在蛋白质分离纯化中的应用[J]. 食品科学, 2003, 24(1): 167-171 |
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| 作者姓名： | 姚红娟 王晓琳 丁宁 |
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| 作者单位： | 清华大学化学工程系,北京,100084 |
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| 基金项目： | 国家自然科学基金资助项目(29876018) |
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| 摘 要： | 以压力差为推动力膜分离过程（过滤，超滤，纳滤，反渗透）的分离性能由透过通量和截留率表征，其操作模式分浓缩和渗滤两种。膜分离技术用于分离，纯化，回收和浓缩蛋白质，如乳清蛋白，血清白蛋白，蛋清蛋白，西蒙德木蛋白，重组白细胞介素－2包涵体以及二元蛋白质混合物等。
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| 关 键 词： | 膜分离 蛋白质 分离 纯化 |
| 文章编号： | 1002-6630(2003)01-0167-05 |
Membrane Separation Technology and Its Application in Purifying and Separating the Proteias |
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| Yao Hongjuan. Membrane Separation Technology and Its Application in Purifying and Separating the Proteias[J]. Food Science, 2003, 24(1): 167-171 |
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| Authors: | Yao Hongjuan |
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| Affiliation: | Yao Hongjuan |
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| Abstract: | Membrane separation technology an d its applications in purifying and s eparating bioproducts were re-viewed.The pressure -driven membra ne processes discussed were microfiltration(MF ),ultrafiltration(UF),nanofiltration(NF),reverse osmosis(RO),which differ fromthe pore sizes.Their separation performances were characterizedbypermeatefluxandr ejection.Membraneseparationprocesses wereoftenoperatedinconcent ration and diafiltration modes and have bee n applied in the fractionation and pu rification of different proteins,s uch as whey protein,serumalbumin,egg whi te protein,Jojoba protein,recombi nant interleukin -2and some binary protein mixtures. |
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| Keywords: | membrane separation microfiltra tion ultrafiltration concentration diafiltration |
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