| 丙型肝炎病毒分片段抗体检测及优势抗原表位分析 |
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| 引用本文： | 谢忠平,杨蓉,龙润乡,李华,白惠株,廖云,蒋蕊鞠,王燕,洪超.丙型肝炎病毒分片段抗体检测及优势抗原表位分析[J].粉末涂料与涂装,2010,23(5). |
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| 作者姓名： | 谢忠平 杨蓉 龙润乡 李华 白惠株 廖云 蒋蕊鞠 王燕 洪超 |
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| 作者单位： | 中国医学科学院北京协和医学院,医学生物学研究所诊断试剂室,昆明,650118 |
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| 基金项目： | 国家高技术研究发展计划(863计划)，云南省联合支持国家计划项目 |
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| 摘 要： | 目的检测丙型肝炎病毒(HCV)分片段抗体,并分析HCV优势抗原表位,为HCV抗原、抗体检测及疫苗研究提供依据。方法收集经ELISA法检测抗HCV阳性血清样品90份,使用总抗体检测试剂及抗HCV分片段试剂进行检测,并用荧光定量PCR对抗体弱阳性及阴性样品进行复核,分析不同片段出现的阳性率,从而分析HCV的优势抗原表位。结果 90份抗HCV阳性的样品经总抗体检测试剂检测,其中阳性78份(86.67%);90份抗HCV阳性的样品中,分片段试剂检测1个片段以上阳性样品59份(65.56%);其中C、NS3、NS4及NS5片段抗体阳性分别为56份(94.92%)、57份(96.61%)、42份(71.19%)及47份(79.66%),NS3及C片段抗体是HCV的优势抗体。结论 HCV-C及NS3表位是HCV的优势抗原表位;HCV分片段抗体与总抗体检测试剂之间阳性检出率存在一定差异。
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| 关 键 词： | 丙型肝炎病毒 分片段抗体 优势抗原表位 |
Detection of HCV Fragmental Antibody and Analysis of Prominent Antigenic Epitope |
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| XIE Zhong-ping,YANG Rong,LONG Run-xiang,et al.Detection of HCV Fragmental Antibody and Analysis of Prominent Antigenic Epitope[J].Chinese Journal of Biologicals,2010,23(5). |
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| Authors: | XIE Zhong-ping YANG Rong LONG Run-xiang |
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| Affiliation: | XIE Zhong-ping,YANG Rong,LONG Run-xiang,et al(Institute of Medical Biology,Chinese Academy of Medical Sciences , Peking Union Medical College,Kunming 650118,China) |
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| Abstract: | Objective To detect HCV fragmental antibody,analyze the prominent antigenic epitope and provide a basis for the detection of antigen and antibody of HCV as well as development of hepatitis C vaccine.Methods Ninety serum specimens proved as anti-HCV positive by ELISA were collected and tested by total anti-HCV detection kit and HCV fragmental antibody detection kit respectively,of which those with weak positive or negative results were retested by fluorescent quantitative PCR.The positive rates of various an... |
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| Keywords: | Hepatitis C virus(HCV) Fragmental antibody Prominent antigenic epitope |
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