| 纳米金与细胞相互作用机理的蛋白质组学研究 |
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| 引用本文： | 吕晓迎,瞿颖华,杨雅敏,黄炎. 纳米金与细胞相互作用机理的蛋白质组学研究[J]. 功能材料, 2011, 42(6): 1016-1020 |
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| 作者姓名： | 吕晓迎 瞿颖华 杨雅敏 黄炎 |
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| 作者单位： | 东南大学,生物电子学国家重点实验室,江苏,南京,210096 |
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| 基金项目： | 国家自然科学基金资助项目(30770583); 国家重点基础研究发展计划(973计划)资助项目(2009CB930000); 教育部高等学校博士学科点专项科研基金资助项目(20100092110027) |
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| 摘 要： | 应用蛋白质组学结合生物信息学方法研究纳米金与人皮肤成纤维细胞(HDF-f )的作用机理.首先采用柠檬酸钠还原氛金酸法制备20nm的纳米金,然后应用MTT法和流式细胞术评价纳米金的细胞毒性及对细胞周期和细胞凋亡的影响.接着应用蛋白质组学技术和生物信息学方法筛选纳米金作用后细胞发生差异表达的蛋白质并进行基因本体论分析.MT...
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| 关 键 词： | 纳米金 蛋白质组学 生物信息学 GO分析 分子生物相容性 |
Proteomic analysis of molecular mechanism of interactions between gold nanoparticles and human dermal fibroblasts-fetal |
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| LV Xiao-ying,QU Ying-hua,YANG Ya-min,HUANG Yan. Proteomic analysis of molecular mechanism of interactions between gold nanoparticles and human dermal fibroblasts-fetal[J]. Journal of Functional Materials, 2011, 42(6): 1016-1020 |
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| Authors: | LV Xiao-ying QU Ying-hua YANG Ya-min HUANG Yan |
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| Affiliation: | LV Xiao-ying,QU Ying-hua,YANG Ya-min,HUANG Yan(State Key Laboratory of Bioelectronics,Southeast University,Nanjing 210096,China) |
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| Abstract: | The aim of the present study is to investigate the mechanism of interactions between gold nanoparticles(GNPs) and human dermal fibroblasts-fetal(HDF-f) based on proteomics technology combining bioinformatics analysis.20nm GNPs was prepared by sodium citrate reducing chloroauric acid at first.Then Methylthiazoltetrazolium(MTT) assay and flow cytomtery were applied to evaluate cytotoxicity and the influence on cell cycle and apoptosis by GNPs.Finally,differentially expressed proteins in HDF-f treated with GNP... |
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| Keywords: | gold nanoparticles proteomics bioinformatics gene ontology molecular biocompatibility |
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