| 地衣芽孢杆菌耐高温α-淀粉酶基因在大肠杆菌中的克隆及表达 |
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| 引用本文： | 蔡恒,陈忠军,路福平,杜连祥. 地衣芽孢杆菌耐高温α-淀粉酶基因在大肠杆菌中的克隆及表达[J]. 食品与发酵工业, 2004, 30(12): 15-18 |
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| 作者姓名： | 蔡恒 陈忠军 路福平 杜连祥 |
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| 作者单位： | 天津科技大学食品科学与生物工程学院,天津,300222 |
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| 摘 要： | 用PCR方法从地衣芽孢杆菌中扩增了耐高温α淀粉酶基因,将扩增的DNA片段插入到大肠杆菌载体pUC19中,构建重组分泌型表达载体pUAM。pUAM中的耐高温α淀粉酶基因在大肠杆菌JM109中得到表达。经SDSPAGE分析显示,蛋白表达产物的分子量为55ku,同核酸序列测定所推导的值相符。
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| 关 键 词： | 地衣芽孢杆菌 耐高温α-淀粉酶 基因克隆与表达 大肠杆菌 |
| 修稿时间： | 2004-08-23 |
Cloning and Expression of the Gene Encoding Heat-stable α-amylase from Bacillus licheniformis in E.coli JM109 |
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| CAI Heng,Chen Zhongjun,Lu Fuping,Du Lianxiang. Cloning and Expression of the Gene Encoding Heat-stable α-amylase from Bacillus licheniformis in E.coli JM109[J]. Food and Fermentation Industries, 2004, 30(12): 15-18 |
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| Authors: | CAI Heng Chen Zhongjun Lu Fuping Du Lianxiang |
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| Abstract: | The heat-stable α-amylase gene was amplified from Bacillus licheniformis by using PCR technique.The amplified 1.9kb DNA fragment was inserted into an E.coli vector pUC19 to yield the recombinant secrete plasmid pUAM.The heat-stable α-amylase gene in Bacillus licheniformis was expressed in E.coli JM109.SDS-PAGE analysis shows that the molecular weight of the expressed recombinent product is about 55 ku,which correspond with the prediction by gene sequence. |
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| Keywords: | Bacillus licheniformis heat-stable α-amylase gene cloning and expression E.coli |
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