Development of New Microsatellite DNA Markers from Apostichopus japonicus and Their Cross-Species Application in Parastichopus parvimensis and Pathallus mollis |
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| Authors: | Liao Meijie Wang Yingeng Rong Xiaojun Zhang Zheng Li Bin Wang Lan Chen Guiping |
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| Affiliation: | Qingdao Key Laboratory for Marine Fish Breeding and Biotechnology, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 106 Nanjing Road, Qingdao 266071, China; E-Mails: liaomj@ysfri.ac.cn (M.L.); rongxj@ysfri.ac.cn (X.R.); zhangzheng@ysfri.ac.cn (Z.Z.); libin841008@163.com (B.L.); wanglan0829@yahoo.cn (L.W.); chengp@ysfri.ac.cn (G.C.). |
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| Abstract: | Twenty microsatellite DNA markers were developed for sea cucumber and used to investigate polymorphisms of 60 wild Apostichopus japonicus individuals collected from China. It revealed that all the markers were polymorphic. A total of 164 alleles were detected at 20 loci. The number of alleles per locus varied from 3 to 17 with an average of 8.2, and the expected heterozygosities of each locus ranged from 0.03 to 0.89 with an average of 0.64. Cross-species amplification was also conducted in Parastichopus parvimensis collected from the United States and Pathallus mollis collected from Peru. The result showed that 17 loci amplified Parastichopus parvimensis DNAs while only 4 loci could amplify Pathallus mollis DNAs. All of the polymorphic markers would be useful for future genetic breeding and the assessment of genetic variation within sea cucumbers. |
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| Keywords: | sea cucumber Apostichopus japonicus Microsatellite DNA marker cross-species amplification |
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