食品中残留19-去甲睾酮免疫原的合成与鉴定

19-去甲睾酮(19-nortestosteroneNT),属于半抗原生物小分子,具有免疫反应性,但不具有免疫原性。本文中19-去甲睾酮经羧甲基羟胺肟化,质谱(MS)检验后,采用混合酸酐法将肟化产物与牛血清蛋白(bovineserumalbuminBSA)偶联,紫外分光光度法测定其交联率为24。用此合成产物进行动物体免疫,获得了NT的多克隆抗体。经间接酶联免疫测定,其抗血清的稀释度可以达到1:102400,证明诺龙免疫原的合成成功,为诺龙酶联免疫试剂盒的研究奠定基础。

Synthesis and Identification of Immunogen 19-nortestosterone Residues in Foods

19-nortestosterone (NT) is a small molecular hapten that has immunoreactivity but hasn’timmunogenicity. In this article, the 19-nortestosterone was oximated by carboxymethoxylamine and identified by MS (mass spectrograph). In order to stimulate the production of antibody, it was coupled with a carrier, bovine serum albumin (BSA),through mixed-anhydride reaction. The conjugate rate of NT and BSA was 24, identified by the analysis of spectrophotometry. Then polyclonal antibody of NT was acquired from animal immunization of this production. The dilute rate of antiserum can reach 1:102400, through an indirect enzyme-linked immunosorbent assay (ELISA), which demonstrates the synthesis of immunogen successful .And this success lays the groundwork for future researches of NT-ELISA kit.