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A Simple and Selective Analytical Procedure for the Extraction and Quantification of Lutein from Tomato By-Products by HPLC–DAD
Authors:Domenico Montesano  Oriella Gennari  Serenella Seccia  Stefania Albrizio
Affiliation:1.Dipartimento di Chimica Farmaceutica e Tossicologica,Università degli Studi di Napoli “Federico II”,Napoli,Italy
Abstract:Lutein, a yellow dihydroxylated carotenoid, is present in many dietary supplements due to its biological properties. Currently, lutein is extracted from marigold flowers by expensive and time-consuming processes. Since tomatoes contain significant levels of lutein, in this study we have examined the feasibility of using tomato by-products as an alternative, low-cost source of this carotenoid. The determination of this carotenoid was performed by high-performance liquid chromatography–diode array detection, after selective extraction from tomato waste product samples. Lutein levels ranged from 9.9 to 10.5 μg/g dry weight. Tomato waste products may be indicated as an alternative commercial source of lutein for food fortification and/or dietary supplements.
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