Application of a specificity ofMucor miehei lipase to concentrate docosahexaenoic acid (DHA) |
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| Authors: | Per Langholz Peter Andersen Torben Forskov Walther Schmidtsdorff |
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| Affiliation: | (1) Technological Laboratory Ministry of Fisheries, Technical University of Denmark, Building 221, DK 2800 Lyngby, Denmark;(2) Department of Chemical Engineering, Technical University of Denmark, Building 229, DK 2800 Lyngby, Denmark |
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| Abstract: | Lipases catalyze the reesterification or hydrolysis of fats and oils and may show specificity towards certain fatty acids. An immobilized lipase from the fungusMucor miehei shows specificity towards docosahexaenoic acid (DHA) (DHA being a poor substrate), whether the DHA is used as a free fatty acid (FFA) as substrate in esterification with methanol, or fatty acid methyl ester (FAME) is used as a substrate for hydrolysis. The specificity of the lipase fromM. miehei may be applied to concentrate DHA originating from a marine oil in either the FAME or the FFA fraction, which can be separated. |
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