| 在线固相萃取富集-高效液相色谱法快速测定花生中白藜芦醇 |
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| 引用本文： | 陈小媚,丁小霞,喻理,王秀嫔,马飞,姜俊,胡小风,李培武. 在线固相萃取富集-高效液相色谱法快速测定花生中白藜芦醇[J]. 中国油料, 2013, 0(6): 712-716 |
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| 作者姓名： | 陈小媚 丁小霞 喻理 王秀嫔 马飞 姜俊 胡小风 李培武 |
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| 作者单位： | [1]中国农业科学院油料作物研究所,湖北武汉430062 [2]农业部油料作物生物学与遗传育种重点实验室,湖北武汉430062 [3]农业部生物毒素检测重点实验室,湖北武汉430062 [4]农业部油料产品质量安全风险评估实验室,湖北武汉430062 [5]农业部油料及制品质量监督检验测试中心,湖北武汉430062 |
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| 基金项目： | 公益性行业（农业）科研专项（201303088）;国家科技支撑计划（2012BAK08803）;“双打”研究专项（2012104010-4）;湖北省自然科学基金（2011CDB356） |
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| 摘 要： | 建立了简便、快速测定花生中白藜芦醇的在线固相萃取富集-高效液相色谱法。固相萃取富集柱填料为dsDNA，在线固相萃取富集过程的上样溶剂为纯水，淋洗溶剂为纯水，转移溶剂为乙腈-水（80：20，V/V），分析测定溶剂为乙腈-水（25：75，V／V）。本方法对白藜芦醇检测的线性方程为Y=1843．805X＋39．1791，相关系数为0．99978；检出限（LOD）为0．004mg／kg；定量限（LOQ）为0．01mg／kg；基质4个水平添加回收率（n=6）在92．48％-107．98％；6次加标回收相对标准偏差（RSD）在0．19％～6．05％。
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| 关 键 词： | 在线固相萃取富集 高效液相色谱法 花生 白藜芦醇 |
Determination of resveratrol in peanut by high performance liquid chromatography spectrometry using on- line solid phase extraction |
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| Affiliation: | CHEN Xiao - mei, DING Xiao - xia, YU Li, WANG Xiu - pin , MA Fei, JIANG Jun, HU Xiao -feng, LI Pei -wu ( 1. Oil Crops Research Institute , Chinese Academy of Agricultural Sciences, Wuhan 430062, China ; 2. Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, China ; 3. Key Laboratory of Detection for Mycotoxins , Ministry of Agriculture, Wuhan 430062, China ; 4. Laboratory of Risk Assessment for Oilseeds Products, Ministry of Agriculture, Wuhan 430062, China ; 5. Quality Inspection and Test Center for Oil seeds and Products, Ministry of Agriculture, Wuhan 430062, China) |
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| Abstract: | A novel method was developed to determine resveratrol in peanut by HPLC with on -line solid phase extraction. The on - line soild phase extraction column was prepared by packing dsDNA column filler in stainless column. The loading and washing solution of the extraction column were both water, the transferring solu- tion was acetonitrile -water （80： 20, V/V）, and the determining solution was acetonitrile -water （25： 75, V/V）. Under the above condition, the linear regress equations was Y = 1 843. 805X ＋ 39. 179 1, with correlation coeffi- cients of 0.999 78, detection limits of 0.004mg/kg, and quantification limits of 0.01mg/＇kg. The average recoveries of 4 levels spiking were 92.48% to 107.98%, the relative standard deviation （RSD） was 0.19% to 6.05% （ n =6）. |
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| Keywords: | On - line solid phase extraction High performance liquid chromatography （ HPLC ） Peanut Res- veratrol |
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