聚乳酸-聚己内酯/丝素蛋白三元复合纳米纤维膜支架的结构与性能

通过静电纺丝技术制备出不同质量比的聚乳酸聚己内酯/ 丝素蛋白（PLA-PCL/SF）复合纳米纤维膜支架，采用扫描电子显微镜、傅里叶红外光谱仪对纳米纤维膜的形貌和结构进行表征，测试纤维膜的孔隙率和吸附性能。结果表明：PLA-PCL与SF 这2 种组分的质量比对复合纳米纤维膜的形貌有显著影响，质量比为90:10和70:30的纳米纤维表面分布着密集的孔洞；PLA-PCL/SF复合纳米纤维膜中SF 经甲醇处理后由无定形结构转变为β?折叠结构；随着复合纳米纤维膜中SF 含量的增加，纳米纤维膜的孔隙率和吸附性也逐渐降低。接触角实验和小鼠胚胎成纤维细胞（NIH-3T3）培养结果表明，SF的加入提高了纳米纤维膜的亲水性，有利于NIH-3T3细胞的黏附和增殖。

Structure and properties of polylactic acid-polycaprolactone/ silk fibroin composite nanofibrous scaffolds

Polylactic acid-polycaprolactone/silk fibroin (PLA-PCL/SF) composite nanofibrous scaffolds with different mass ratios were fabricated by electrospinning.The morphologies and structures of nanofibrous mats were characterized by scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FT-IR).The porosity and adsorbent behavior of the fibrous mats were also measured.The results shows that the mass ratio of PLA-PCL and SF components has obvious influence on the morphologies of nanofibrous scaffolds,and high density pores present on nanofibrous surfaces at mass ratios of 90∶10 and 70∶ 30.The structure of SF in PLA-PCL/SF composite scaffold is changed from amorphous structure to β-sheet conformation by methanol treatment.The porosity and adsorbent behavior of the nanofibrous mats decrease with increasing SF contents in composite nanofibrous scaffolds.The water contact angle test and mouse fibroblasts cells (NIH-3T3) culture results indicate that the hydrophilicity of nanofibrous scaffolds is improved by the addition of SF for better adhesion and proliferation of NIH-3T3 cells.