人脐静脉内皮细胞形貌与表面粘附力的原子力显微镜表征

目的:探讨原子力显微镜(AFM)在研究人脐静脉内皮细胞(ECV304)表面形貌、超微结构及纳米机械性质等方面的应用,讨论ECV304超微结构和机械性质与其功能的关系。方法:利用AFM对ECV304细胞的表面形貌及生物机械性质进行表征与测量。结果:在AFM下观察到用普通光学显微镜难以观察到的ECV304细胞的独特的形态结构,如细胞骨架、伪足及细胞边缘微丝等。ECV304细胞呈现长梭形、多角形、圆形等多种形态,细胞表面平均粗糙度为320.52±75.98 nm,表面均匀分布微绒毛,细胞周围有铺展的圆盘状物质。力曲线定量分析得出针尖与细胞表面的非特异性粘附力为75±14 pN。结论:通过AFM成像和力曲线测量表明,ECV304细胞呈圆形,多角形,梭形等多种形态,针尖与细胞膜表面问的粘附力比较小,约75±14pN。

Atomic force microscopy-based analysis of morphological and adhesion force of ECV304 cells

Aim:To detect the morphological and nanomechanical properties of ECV304 cells using atomic force microscopy(AFM).Method:The morphology of ECV304 cells was obtained using imaging mode of AFM and the mechanical properties were characterized by measuring force-distance curves.Results:Using AFM,the structure of ECV304 cell such as cytoskeleton,pseudopod,microfilament,etc.can be observed,and these structures were difficult to be obtained by other conventional optical microscopy.ECV304 cells present multiple shapes and there is uniformly distribution of microvilli on the cell surface.The average roughness of cell membrane is 320.52 ±75.98 nm which indicated the cell surface was not smooth.The force-distance curves measurements demonstrate the adhesion force between AFM tip and ECV304 cell was 75±14 pN and this data will give a quantitative reference to distinguish the biophysical conditions of ECV304 cell.