食品中若干植物源性成分的PCR检测

采用CTAB法提取豆奶粉、马铃薯、玉米、番茄及其制品样品中的总DNA,通过PCR方法检测大豆lectin、马铃薯patatin、玉米IVR、番茄PG等特异性内源基因,判断食品中是否含有大豆、马铃薯、玉米、番茄等植物源性成分。同时还进行了这些内源基因分别与核糖体18SrDNA或叶绿体rbcL基因之间的二重PCR分析。结果表明单PCR与二重PCR分析方法可靠稳定,可应用于食品中的植物源性成分的鉴定。

PCR Detection for Some Components Extracted from Plant in Food

Total DNA was extracted from soybean-milk powder and potato, maize, tamato and their procced food. The plant derived components in food were identified by PCR detection for plant endogenous genes such as soybean lectin gene, potato patatin gene, maize IVR gene and tomato PG gene as well as their command genes such as rbcL gene of plant chloroplast and 18S rDNA fragment. Duplex PCR of above very endogenous gene together with 18S rDNA or rbcL gene respectively were also carded out. The results indicated single PCR and duplex PCR could be applied to identify plant derived components in food steadily.