| 酸法提取斑点叉尾鮰鱼皮胶原工艺的条件优化 |
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| 引用本文： | 刘海涛,陶玉贵,陈静颖,王其进,谷浩. 酸法提取斑点叉尾鮰鱼皮胶原工艺的条件优化[J]. 安徽机电学院学报, 2010, 0(2): 22-25 |
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| 作者姓名： | 刘海涛 陶玉贵 陈静颖 王其进 谷浩 |
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| 作者单位： | 安徽工程大学生物与化学工程学院,安徽芜湖241000 |
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| 基金项目： | 安徽省自然科学研究重点基金资助项目（kj2008a078） |
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| 摘 要： | 采用酸法从人工养殖的鮰鱼皮中提取胶原,并研究了酸的种类、酸的浓度、料液比、提取时间和提取温度对胶原提取率的影响.正交实验研究结果表明,最适提取工艺条件为：乙酸浓度1.0 mol/L,固液比1∶30,提取时间72 h,在5℃下提取得率为33.79%,提取胶原的最大紫外吸收波长为236 nm,和标准品紫外吸收波长基本一致.
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| 关 键 词： | 鮰鱼皮 胶原 酸法 提取 |
Optimization of the extraction of collagen with acid from the skin of Ictalurus punctatus |
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| LIU Hai-tao,TAO Yu-gui,CHEN Jing-ying,WANG Qi-jin,GU Hao. Optimization of the extraction of collagen with acid from the skin of Ictalurus punctatus[J]. Journal of Anhui Institute of Mechanical and Electrical Engineering, 2010, 0(2): 22-25 |
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| Authors: | LIU Hai-tao TAO Yu-gui CHEN Jing-ying WANG Qi-jin GU Hao |
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| Affiliation: | (Coll.of Bioch.Engn.,Anhui Polytechnic University,Wuhu 241000,China) |
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| Abstract: | The technology of extracting collagen from the skin of Ictalurus punctatus is studied in the paper.The extraction technology includes acid choice,acid concentration,immersion time and temperature.The optimal processing conditions were obtained by orthogonal experiments.The parameters were as follows：concentration of acetic acid is 1.0 mol/L and the solid-liquid ratio is 1∶30,immersion time is 72 h at 5 ℃,the recovery of the collagens was 33.79%.Collagen from the skin of Ictalurus punctatus absorb light with a λmax of 236 nm,with the standard UV absorption wavelength of being almost the same. |
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| Keywords: | skin of Ictalurus punctatus collagen acid solution extraction |
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