有机碳源添加对脱氮副球菌脱氮效果的影响

以脱氮副球菌(Paracoccus denitrificans)为菌种来源,可生物降解聚合物PCL为有机碳源和生物膜载体,对养殖水体的硝酸盐氮进行脱除实验。结果表明,以特定反硝化菌株接入反硝化装置,可以有效去除硝酸盐氮和亚硝酸盐氮。经过15 d的驯化,60 d的反硝化实验,硝酸盐氮的去除率达到79%,且无亚硝态氮的明显累积;扫描电镜结果表明,固相碳源表面形成的凹陷可为脱氮副球菌提供碳源及载体,具有较好的生物利用性。以PCL为碳源,可以提高养殖水体中的C/N,且操作简单,在经济上具备一定的可行性。

Effect of Organic Carbon Source on the Nitrogen Removal of Paracoccusdenitrificans

In the experiment of removing nitrate in aquaculture water,Paracoccusdenitrificanswas used as bacteria sources,while PCL in the form of biodegradable polymer pellets(BDPs)was used as a carbon source and a biofilm carrier.The experimental results indicated that nitrate and nitrite nitrogen were effectively removed when specific denitrifying strainswere inoculated into the denitrifying device.After 15 d of domestication and 60 dof denitrification,the removal rate of nitrate nitrogen reached 79%,and there was no significant accumulation of nitrous nitrogen;Scanning electron Microscope(SEM)results showed that depressions formed on the surface of PCL,which includinggood bioavailability,could provide carbon sources and carriers for P.denitrificans.Therefore,PLC applied as carbon source can improve C/N in aquaculturewater,and its operation is comparatively simple and economical.