卷烟添加木犀草素与黄芩苷混合物对细胞损伤的减害作用研究

将木犀草素、黄芩苷混合物加入卷烟制成样品卷烟,研究其在卷烟烟气中对人肺泡上皮细胞损伤的影响。用不同浓度卷烟烟气提取物(cigarette smoke extract,CSE)刺激人肺泡上皮细胞A549,采用MTT实验检测细胞存活率,彗星实验观察DNA损伤,荧光法测定细胞内活性氧(reactive oxygen species,ROS)含量。结果表明,与对照卷烟比较,样品卷烟组细胞存活率升高,细胞内活性氧(ROS)彗星尾长、尾部DNA含量、尾距、Olive尾距均小于对照卷烟组。因此,添加木犀草素与黄芩苷混合物的特制卷烟烟气对细胞的损伤较少,产生较少ROS,减少一定的DNA损伤,对细胞毒性有一定的减害作用。

Effects of adding Luteolin and Baicalin mixture in cigarettes on reducing cell injury

Sample cigarettes were made by adding luteolin and baicalin mixture into cigarettes.Cell cytotoxicity experiments were conducted.Cigarette smoke extract(CSE) was collected by dimethyl sulfoxide(DMSO).Human lung adenocarcinoma A549 cells were cultured and then incubated with different concentrations of CSE.Cell viability was evaluated by MTT assay and DNA damage was detected by Comet assay.Contents of reactive oxygen species(ROS) was examined by fluorescence method.Results showed that the sample cigarettes resulted in higher survival rate of A549 cells,lower ROS tail length,tail DNA content,TM,and OTM than control group.It implied that adding luteolin and baicalin mixture into cigarettes could attenuate cell injury,increase cell viability,and reduce DNA damage.