3H11的~(123)I标记及其生物分布

采用Iodogen氧化法对胃癌单克隆抗体3 H11进行了123I标记,用PD-10层析柱分离纯化标记物,纸层析法测定标记物的标记率和放化纯度,评价标记物的体外稳定性,并观察了标记物在正常小鼠体内的生物分布。标记结果显示,123I-3 H11的优化标记条件为:Iodogen 10μg、3 H11 30μg、Na123I溶液20μL(13.3 MBq)、磷酸盐缓冲溶液100μL(pH7.4、0.2 mol/L)、常温下反应8 min,123I-3 H11标记率70%~80%;稳定性结果显示,标记物在4℃人血清中的体外稳定性较好,放置48 h后放化纯度92%;正常昆明鼠体内生物学分布显示,全抗3 H11血液半清除时间为12.25±0.25 h,胃组织有明显摄取。以上结果提示,123I-3 H11是一种很有前景的肿瘤放射免疫显像剂。

Labeling of 3H11 With 123I and Its Biodistribution

3H11 was labeled with 123I by Iodogen method,and the labeling product were purified with PD-10 column.The labeling yield and the radiochemical purity of the product was determined by paper chromatography.The biodistribution of 123I-3H11 in normal mice was carride out as well.The optimal experimental conditions of 123I-3H11 was as follow： Iodogen 10 μg,3H11 30 μg,Na123I solution 20 μL（13.3 MBq）,PBS 100 μL（pH 7.4,0.2 mol/L）,the normal temperature for 8 min.The labeling yield of 123I-3H11 was 70%-80%.After stored at 4 ℃ for 48 h in human serum,the radiochemical purity was more than 92%.The results of biodistribution showed that the clearance of radiolabeled antibody in blood（half time,T1/2） was 12.25±0.25 h,and the radioactivity in the stomach was uptaken obviously.The above results indicated that 123I-3H11 appears to show some potential as gastric cancer imaging diagnostic agent.