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| 脂肪酶热稳定性改造研究进展 | |
| 引用本文： | 谭中标,李剑芳,邬敏辰,殷欣,胡蝶,董运海. 脂肪酶热稳定性改造研究进展[J]. 食品与生物技术学报, 2014, 33(7): 673-681 |
| 作者姓名： | 谭中标 李剑芳 邬敏辰 殷欣 胡蝶 董运海 |
| 作者单位： | 1. 江南大学生物工程学院,江苏无锡,214122 2. 江南大学食品学院,江苏无锡,214122 3. 江南大学无锡医学院,江苏无锡,214122 |
| 摘 要： | 热稳定性改造是脂肪酶酶学性质研究的热点之一,随着技术的发展,越来越多的有效手段被用于脂肪酶热稳定性改造。作者综述了影响脂肪酶热稳定性的主要因素、提高其热稳定性的研究方法以及最新研究成果,为脂肪酶的进一步改造提供依据,并对其他酶的酶学性质改造具有借鉴意义。 |
| 关 键 词： | 脂肪酶 热稳定性 随机突变 定向进化 定点突变 |
Research Advance on Engineering Thermostability of Lipase |
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| TAN Zhongbiao,LI Jianfang,WU Minchen,YIN Xin,HU Die and DONG Yunhai. Research Advance on Engineering Thermostability of Lipase[J]. Journal of Food Science and Biotechnology, 2014, 33(7): 673-681 | |
| Authors: | TAN Zhongbiao LI Jianfang WU Minchen YIN Xin HU Die DONG Yunhai |
| Affiliation: | TAN Zhongbiao;LI Jianfang;WU Minchen;YIN Xin;HU Die;DONG Yunhai;School of Biotechnology,Jiangnan University;School of Food Science and Technology,Jiangnan University;Wuxi Medical School,Jiangnan University; |
| Abstract: | Engineering thermostability of lipase is a hot topic of improving properties of the enzyme. With the development of technology, more and more effective means are used in engineering thermostability of lipase. This paper summarizes the main factors influencing the thermostability of lipase, methods to improve it and the latest research results on it. It could provide a basis for further research on lipase and a reference for altering character of other enzymes. |
| Keywords: | lipase thermostability random mutagenesis directed evolution site-directed mutagenesis |
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