Structural Characterization of Neutral Glycosphingolipids from 3T3‐L1 Adipocytes |
| |
| Authors: | Hisao Kojima Yusuke Suzuki Masahiro Ito Kazuya Kabayama |
| |
| Affiliation: | 1. , College of Life Science, Ritsumeikan University, Shiga, Japan;2. , College of Science and Technology, Nihon University, Tokyo, Japan;3. , Graduate School of Science, Osaka University, Toyonaka, Osaka, Japan |
| |
| Abstract: | In recent years, obesity has been considered a pathological stage of early lifestyle‐related diseases, and adipose tissue and adipocyte research has been active. Glycosphingolipids are involved in the pathogenesis of type 2 diabetes induced by insulin resistance, but the details of the glycosphingolipid molecular species composition of adipocytes have yet to be elucidated. We used 3T3‐L1 adipocytes and the 1,2‐dichloroethane‐wash method to remove triacylglycerols, which are abundant in adipocytes, and analyzed the structures of glycosphingolipids, particularly neutral glycosphingolipids, using liquid chromatography–mass spectrometry. |
| |
| Keywords: | Glycosphingolipids 1,2‐Dichloroethane wash Adipocytes Triacylglycerols Liquid chromatography– tandem mass spectrometry |
|
|
