Incorporation of Alpha‐Linolenic Acid and Enhancement of n‐3 Fatty Acids in Nile Tilapia: a Factorial Design |
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Authors: | Vanessa Vivian Almeida Schneider Fabiana Carbonera Paula Fernandes Montanher Ana Paula Lopes Makoto Matsushita Jesuí Vergílio Visentainer |
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Affiliation: | 1. +554430113661;2. Federal University of Technology‐Paraná, Apucarana, PR, Brazil;3. , Post‐Graduate Program of Food Science, State University of Maringá, Maringá, PR, Brazil;4. , Department of Chemistry, State University of Maringá, Maringá, PR, Brazil |
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Abstract: | A 22 factorial design (two factors at two levels, in triplicate) was performed to investigate the influence of factors A (time of treatment, 15 and 30 days) and B (chia oil content in a supplemented diet, at 2.1 and 4.2 %) in three responses of interest referring to: (a) the incorporation of alpha‐linolenic acid (LNA) in lipids of Nile tilapia fillet; (b) the enhancement of n‐3 fatty acids; and (c) the decrease in the omega‐6/omega3 (n‐6/n‐3) ratio in fish. Factors A and B were significant in the three regression models obtained and the interaction AB was a significant contributor to the LNA and n‐6/n‐3 ratio. Analysis of variance suggested three significant and predictive mathematical models. Response surfaces analyses from designs indicated higher LNA and n‐3 contents and a lower n‐6/n‐3 ratio using both factors A and B in the higher levels (30 days of treatment and 4.2 % of chia oil in the diet for fish) chosen for this study. |
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Keywords: | Salvia hispanica L Oreochromis niloticus Omega‐3 Supplemented diet Chemometrics n‐6/n‐3 ratio Chia oil |
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