Improved lycopene extraction from tomato peels using cell-wall degrading enzymes |
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Authors: | Roberto Lavecchia Antonio Zuorro |
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Affiliation: | (1) Department of Chemical Engineering, Materials and Environment, Sapienza University of Rome, Via Eudossiana 18, 00184 Rome, Italy |
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Abstract: | Four commercial enzyme preparations with pectinolytic, cellulolytic and hemicellulolytic activities were tested for their
ability to enhance lycopene extraction from tomato peels. Screening experiments were performed at 25 °C by subjecting the
peels to a 4-h enzyme incubation followed by 1-h hexane extraction. Peclyve EP and LI were the most efficient, with an almost
20-fold increase in extraction yield. Peclyve LI was used to evaluate the influence of solvent type and enzyme incubation
time on lycopene recovery. Hexane, ethyl acetate and the mixture hexane/acetone/ethanol 50:25:25 (v/v) were used as solvents.
Under the best extraction conditions (1-h enzyme incubation followed by a 3-h solvent extraction at 40 °C) up to 440 mg of
lycopene per 100 g of dry tomato peels were obtained. The percentage recoveries were in the range of 3–30%, for the untreated
peels, and 77–98% for the enzymatically treated material. |
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Keywords: | Enzymes Pectinase Lycopene Tomato peels Tomato waste Solvent extraction |
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