营养条件对产烃葡萄藻生长的影响

在摇瓶培养中考察了无机碳源、氮源、磷源和NaF等营养成份对产烃葡萄藻(Botry℃℃cus braunii)生长的影响. 结果表明：与仅利用空气中的CO2作为碳源相比，以NaHCO3或CO2加富空气补碳，比生长速率从0.0535 d-1分别增大至0.0589和0.0949 d-1，代时从5.6 d分别缩短至5.1和3.2 d，最大放氧速率分别提高了2.3和5倍；在一定范围内增大KNO3起始浓度能提高葡萄藻的生长速率，延长对数生长期；磷源对葡萄藻生长的影响十分显著，在0~160 mg/L范围内，增大K2HPO4浓度，葡萄藻生长明显加快；适量NaF能促进葡萄藻的光合作用和呼吸作用，从而加快葡萄藻的生长，其最适浓度为0.84 mg/L.

Effect of Nutrient Conditions on the Growth of Botryococcus braunii

The effect of nutrient conditions, including incarbon sources, nitrate and phosphate, on the growth of hydrocarbon-rich microalga Botryococcus braunii was investigated in shake flask culture. The complement of NaHCO3 and CO2 made growth rate of Botryococcus braunii increase from 0.0535 d-1 to 0.0589 d-1, 0.0949 d-1 respectively, generation time reduced from 5.6 d to 5.1 d, 3.2 d respectively, maximum oxygen evolution rate improved 2.3 and 5 times respectively by comparison with control cultures. The growth rate of Botryococcus braunii went up and the exponential phase lengthened with the increase of the initial concentration of KNO3 within some scope. K2HPO4 in the range of 0~160 mg/L could improve the growth rate of Botryococcus braunii significantly. In addition, the role of NaF was examined. NaF promoted the photosynthesis and respiration activity. Its suitable concentration was 0.84 mg/L.