A simple and sensitive DNA hybridization assay used for the routine diagnosis of human parvovirus B19 infection |
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| Authors: | KE Hicks S Beard BJ Cohen JP Clewley |
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| Affiliation: | Virus Reference Division, Central Public Health Laboratory, London, United Kingdom. |
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| Abstract: | A dot blot hybridization assay for parvovirus B19 diagnosis was developed by using a PCR-generated probe, digoxigenin labelling, and chemiluminescence detection. Different labelling techniques and hybridization solutions were evaluated. From this analysis a protocol was devised for routine diagnostic use. The protocol enabled 1 pg of B19 DNA to be detected. The results of applying this method to 8,369 diagnostic samples collected during 1994 and 1995 are given. |
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