曝气式反应器中木质素过氧化物酶的合成及对印染废水配制液的脱色处理

比较了黄孢原毛平革菌在3种生物反应器(搅拌式反应器、鼓泡式反应器、曝气式反应器)中合成木质素过氧化物酶(LiP)和锰过氧化物酶(MnP)的差异. 结果表明，曝气式反应器对酶的合成(尤其是LiP)最为有利. 考察了曝气式反应器中半连续培养和连续培养两种方式下酶的合成和橙I脱色情况，发现半连续培养可使培养体系长时间保持较高酶活力，置换比例为1/2时染料废水可连续脱色5批，脱色率达到90%以上，比脱色率在46.7 g/(g×d)以上. 连续培养条件下酶很快失活，废水的脱色率迅速下降. 在曝气式反应器中用半连续培养的方式(置换比例1/2)对实际印染废水进行处理，可处理废水4批，前3批脱色率达到90%以上，第4批有明显下降.

Synthesis of Lignin Peroxidase and Decoloration of Model Printing Wastewater in the Aeration Reactor

Production of lignin peroxidase (LiP) and manganese peroxidase (MnP) in three kinds of reactors (stirred tank reactor, bubble column reactor and aeration reactor) by Phanerochaete chrysosporium was studied. High activity of enzymes, especially for LiP, was obtained in the aeration reactor. The production of enzymes and decoloration of orange I were investigated in semi-continuous culture and continuous culture respectively. High activity of enzymes for a long period of time was detected in the semi-continuous culture, in which five batches of dye wastewater could be decolored to more than 90% and the specific decloration rate of orange I was above 46.7 g/(gd) when the proportion of replacement was 1/2, while enzymes decayed rapidly and poor decoloration was gained in the continuous culture. The application of semi-continuous culture in the aeration reactor was tried for the decoloration of four batches of printing-wastewater, and the decelerations rate of the first three was all above 90%.