Corneal epithelialisation on surface-modified hydrogel implants: artificial cornea |
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Authors: | Ma Aihua Zhao Bojun Bentley Adam J Brahma Arun MacNeil Sheila Martin Francis L Rimmer Stephen Fullwood Nigel J |
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Affiliation: | (1) School of Health and Medicine, Biomedical and Life Sciences, Lancaster University, Lancaster, UK;(2) Centre for Biophotonics, Lancaster Environment Centre, Lancaster University, Lancaster, UK;(3) Manchester Royal Eye Hospital, Manchester, UK;(4) Department of Engineering Materials, The Kroto Research Institute, North Campus, University of Sheffield, Broad Lane, Sheffield, UK;(5) Department of Chemistry, University of Sheffield, Sheffield, UK; |
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Abstract: | The objective was to investigate corneal re-epithelialisation of surface-modified polymethacrylate hydrogel implants in order
to evaluate them as potential materials for an artificial cornea. Polymethacrylate hydrogels were modified with amines and
then coated with different extracellular matrix proteins (collagen I, IV, laminin and fibronectin). The modified hydrogels
were surgically implanted into bovine corneas maintained in a 3-D culture system for 5 days. The epithelial growth across
the implant surface was evaluated using fluorescent, light and electron microscopy. Full epithelialisation was achieved on
1,4-diaminobutane-modified hydrogels after coating with collagen IV. Hydrogels modified with 1,4-diaminobutane but without
further coating only showed partial re-epithelialisation. Hydrogels modified with other amines (1,2-diaminoethane or 1,3-diaminopropane)
showed only partial re-epithelialisation; further coating with extracellular matrix proteins improved epithelialisation of
these surfaces but did not result in complete re-epithelialisation. Evaluation of the corneas implanted with the 1,4-diaminobutane-modified
hydrogels coated with collagen IV showed that the artificial corneas remain clear, integrate well and become covered by a
healthy stratified epithelium. In conclusion the 1,4-diaminobutane surface-modified hydrogel coated with collagen IV supported
the growth of a stable stratified epithelium. With further refinement this hydrogel has the potential to be used clinically
for an artificial cornea. |
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