Characterization of proteolysis in muscle tissues of sea cucumber <Emphasis Type="Italic">Stichopus japonicus</Emphasis> |
| |
Authors: | Chen-Chen Zhao Yang Yang Hai-Tao Wu Zhi-Mo Zhu Yue Tang Cui-Ping Yu Na Sun Qiang Lv Jia-Run Han Ao-Ting Li Jia-Nan Yan Yue Cha |
| |
Affiliation: | 1.School of Food Science and Technology,Dalian Polytechnic University,Dalian,China;2.National Engineering Research Center of Seafood,Dalian,China |
| |
Abstract: | The proteolysis in muscle tissues of sea cucumber Stichopus japonicus (sjMTs) was characterized. The proteins from sjMTs were primarily myosin heavy chains (MHCs), paramyosin (Pm), and actin (Ac) having a molecular mass of approximately 200, 98, and 42 kDa, respectively. Based on SDS-PAGE analysis and quantification of trichloroacetic acid (TCA)-soluble peptides released, degradation of muscle proteins from sjMTs was favorable at pH 5 and 50°C. Proteolysis of MHCs was mostly inhibited by cysteine protease inhibitors, including trans-epoxysuccinyl-L-leucyl-amido (4-guanidino) butane (E-64) and antipain (AP). E-64 and AP completely inhibited the degradation of Pm and Ac, while iodoacetic acid showed a partially inhibitory effect. These results indicated that the proteolysis of sjMTs was mainly attributed to cysteine proteases. Avoidance of setting the tissues at 40–50°C and slightly acidic condition and inhibition of cysteine proteases are helpful for decreasing sea cucumber autolysis. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|