Analysis of interaction between liposome membranes induced by stress condition: Utilization of liposomes immobilized on indium tin oxide electrode |
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Authors: | Haruyuki Ishii Toshinori Shimanouchi Hiroshi Umakoshi Ryoichi Kuboi |
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Affiliation: | aDepartment of Chemical Science and Engineering, Graduate School of Engineering Science, Osaka University, 1-3 Machikaneyama-cho, Toyonaka, Osaka 560-8531, Japan |
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Abstract: | NBD-cholesterol (NBD-Ch)-modified liposome was immobilized on indium tin oxide (ITO) electrode via the covalent binding method. The transfer of NBD-Ch between the immobilized liposomes and the target liposomes was observed by using a fluorescent microscope. The addition of liposome suspension co-incubated with α-chymotrypsin or stimuli-responsive polymer to the surface of the above ITO electrode, enhanced the liposome–liposome interaction, resulting in the promotion of NBD-Ch transfer. The apparent transfer rate constant of NBD-Ch was found to be correlated with the index for the liposome–liposome interaction evaluated by an immobilized liposome chromatography. This suggests that the present method using the liposome-immobilized ITO electrode was effective to evaluate the liposome–liposome interaction induced by the protein or the stimuli-responsive polymer under stress conditions. |
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Keywords: | Immobilization of liposome NBD-cholesterol α -Chymotrypsin Stimuli responsive copolymer Fluorescence microscope |
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