碱性蛋白酶水解脱除蛋白的橡子淀粉分离工艺条件优化

为优化碱性蛋白酶水解蛋白的橡子淀粉提取工艺条件,选择酶解时间、温度、pH值、酶用量等因素进行单因素试验和正交试验,确定了适宜的橡子淀粉提取工艺条件。结果表明,碱性蛋白酶水解去除蛋白的pH值和酶解温度对橡子淀粉中总淀粉含量的影响较大,影响程度依次为pH值〉酶解温度〉酶解时间〉酶用量;碱性蛋白酶水解去除蛋白的适宜工艺条件为pH值为11、酶解温度为45℃、酶解时间为140 min、酶用量为600 U/g。结合1%双氧水漂白处理12 h后,橡子淀粉中的蛋白未检出。

Condition Optimization of Acorn Starch Extraction by Alkali Protease Enzymolysis

To optimization the extraction technology of acorn starch,enzymolysis time,pH value,temperature,enzyme dosage of alkalinity protease,were selected to take orthogonal experiment of conditions of starch extraction by alkaline protease enzymolysis.The results showed that,the affecting factors by alkaline protease enzyme treatment were pH value,enzymolysis temperature,enzymolysis time,and enzyme dosage of alkali protease.The optimization of process conditions by enzymolysis using alkali protease is that the pH is 11,the enzymolysis temperature is 45 ℃,the enzymolysis time is 140 minutes,enzyme dosage is 600 U/g,the content of protein in the starch is 2.32%,the west starch obtained after enzymolysis is soda-wash bleached when the mass rate to starch and water is 1：5,the concentration of aquae hydrogen dioxide is 1%,the pH is 11,temperature is 45 ℃ and time is 12 hours,protein residues in the final starch is not examined.