| 高产蛋白酶活性的枯草芽孢杆菌筛选及鉴定 |
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| 引用本文： | 程德勇,缪礼鸿. 高产蛋白酶活性的枯草芽孢杆菌筛选及鉴定[J]. 武汉工业学院学报, 2014, 0(1): 6-10 |
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| 作者姓名： | 程德勇 缪礼鸿 |
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| 作者单位： | 武汉轻工大学生物与制药工程学院,湖北武汉430023 |
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| 摘 要： | 通过透明圈法从土壤中筛选出20株能分泌胞外蛋白酶的芽孢杆茵，并使用福林-酚试剂法测定了其中透明圈（H／C）值较大的6株和三种市购枯草芽孢杆茵发酵茵液的中性蛋白酶活性。选择中性蛋白酶活性最强的CDY-5菌株（23．44U／mL）及透明圈（H／C）值最大的CDY-1茵株（3．75），进行16SrRNA基因测序后，通过MEGA软件构建系统发育树得出菌株CDY-1为巨大芽孢杆茵，CDY-5为枯草芽孢杆茵。
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| 关 键 词： | 枯草芽孢杆茵 蛋白酶活性 筛选 鉴定 |
Screening and identification of high yield protease producing Bacillus strains |
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| CHENG De-yong,MIAO Li-hong. Screening and identification of high yield protease producing Bacillus strains[J]. Journal of Wuhan Polytechnic University, 2014, 0(1): 6-10 |
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| Authors: | CHENG De-yong MIAO Li-hong |
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| Affiliation: | ( School of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan 430023, China) |
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| Abstract: | Abstract ：Twenty extracellular proteases producing Bacillus strains were screened and identified from soil using transparent circle method. Six of them showed larger transparent circle diameter and other three commercially avail- able Bacillus strains were further inoculated in fermentation culture, and the protease activity in the supematant were detected by forint phenol reagent. Sequencing the 16 S rRNA of the strains showed the highest neutral protease activity CDY-5 （23.44 U/mL） and the largest transparent circle diameter CDY-1 （3.75）, and the phylogenetic trees were constructed by MEGA software. The results showed that the strain CDY-1 and CDY-5 were phylogeneti- cally related to Bacillus megaterium and Bacillus subtilis respectively. |
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| Keywords: | Bacillus subtilis protease activity screened identification |
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