| 免培养技术对浓香型白酒大曲中细菌多样性的影响 |
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| 引用本文： | 汤斌,刘金英,周庆武,李安军,万春环,汤有宏. 免培养技术对浓香型白酒大曲中细菌多样性的影响[J]. 食品与发酵工业, 2011, 37(9) |
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| 作者姓名： | 汤斌 刘金英 周庆武 李安军 万春环 汤有宏 |
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| 作者单位： | 1. 安徽工程大学微生物发酵安徽省工程技术研究中心,安徽芜湖,241000
2. 安徽古井贡酒股份有限公司,安徽毫州,236826 |
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| 基金项目： | 安徽省自然科学基金(KJ2007A018) |
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| 摘 要： | 采用免培养(culture independent)技术直接从浓香型白酒大曲中提取细菌微生物基因组DNA,利用细菌16S rDNA通用引物扩增大曲混合微生物的16S rDNA,采用PCR扩增技术、分子克隆技术以及序列同源性分析等方法测定大曲中细菌的16S rDNA基因全序列,通过与基因数据库中相似菌群序列同源性的比较,构建系统发育树。结果显示,成熟的大曲中细菌共分为Lactobacillus,Pantoea,Enterobacter,Klebsiella,Leuconostoc,Erwinias,Pseudomonas,Bacillus licheniformis几大类群,表现出高度的细菌多样性。
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| 关 键 词： | 大曲 微生物多样性 免培养法 系统发育树 |
Phylogenetic Diversity Analyse of Bacteria in Gujing-flavor Liquor Daqu Using Culture Independent Method |
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| Tang Bin,Liu Jin-ying,Zhou Qing-wu,Li An-jun,Wan Chun-huan,Tang You-hong. Phylogenetic Diversity Analyse of Bacteria in Gujing-flavor Liquor Daqu Using Culture Independent Method[J]. Food and Fermentation Industries, 2011, 37(9) |
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| Authors: | Tang Bin Liu Jin-ying Zhou Qing-wu Li An-jun Wan Chun-huan Tang You-hong |
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| Affiliation: | Tang Bin1,Liu Jin-ying1,Zhou Qing-wu2,Li An-jun2,Wan Chun-huan2,Tang You-hong2 1(Anhui Polytechnic University,Engineering Technology Research Center of Anhui Microbial Fermentation,Wuhu 241000,China) 2(Anhui Gujing Distillery Company Limited,Haozhou 236826,China) |
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| Abstract: | Culture independent method was used to study the diversity of Daqu bacteria.The techniques including culture independent approach,PCR technique and 16S rDNA sequence homology analysis etc.The total DNA directly extracted from Daqu was used as PCR template.Random clones,containing almost full size 16S rDNA sequences(about 1.5 kb long),were sequenced and subjected to an on line similarity search.According to the sequences alignment and through the analysis of its comparability,the bacteria of Daqu were consis... |
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| Keywords: | Gujing-flavor Liquor Daqu microbiology diversity culture independent approach phylogenetic analysis |
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