三株植物乳杆菌降胆固醇机理的研究

测定了已筛选出的具有较高胆固醇去除能力和胆盐水解酶活性的3株植物乳杆菌(KLDS6.0330,KLDS6.0333,KLDS6.0335)的胆固醇同化作用,胆汁盐的去结合作用和胆固醇共沉淀作用。这3株植物乳杆菌能够同化较多的胆固醇(﹥43μg/mL);所有的菌株都表现出了对甘氨胆酸钠和牛磺胆酸钠的去结合作用,与牛磺胆酸钠相比,甘氨胆酸钠的去结合能够释放较多的胆酸;所有菌株都表现出了胆固醇与甘氨胆酸钠和牛磺胆酸钠去结合释放胆酸的共沉淀,与牛磺胆酸钠相比,甘氨胆酸钠去结合产生的胆酸表现出了与较多的胆固醇共沉淀;随着pH值的下降,解聚态的胆汁酸与胆固醇的共沉淀量增加,结合态胆盐与胆固醇的共沉淀量较少。结果表明这3株植物乳杆菌在体外可以通过3种机制去除胆固醇。

Study on the Cholerterol-reducing Mechanism of Three Lactobacillus plantarum Strains

Three strains of Lactobacillus plantarum(KLDS6.0330,KLDS6.0333,KLDS6.0335) showing higher Cholesterol removal ability and bile salt hydrolase activity had screened,we determined its cholesterol assimilation,bile salt deconjugation and cholesterol co-precipitating properties.Three strains of Lactobacillus plantarum can assimilate more choleserol(﹥43μg/mL);All strains exhibited deconjugation of both sodium glycocholate and sodium taurocholate,More cholic acid was liberated from the deconjugation of sodium glycocholate than sodium taurocholate;Co-precipitation of cholesterol with cholic acid was observed from deconjugation of both sodium glycocholate and sodium taurocholate,and by all strains studied.More cholesterol was precipitated with cholic acid when sodium glycocholate was used compared to sodium taurocholate.Increased cholesterol co-precipitation with deconjugated bile was observed with decreasing pH levels,small amount of cholesterol co-precipitated with sodium glycocholate and sodium taurocholate.The results indicated that these three strains of Lactobacillus plantarum could remove cholesterol in vitro via three mechanisms.