| 大豆卵磷脂DNA的提取方法及应用 |
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| 引用本文： | 韩建勋,吴亚君,王斌,杨海荣,陈颖. 大豆卵磷脂DNA的提取方法及应用[J]. 食品与发酵工业, 2011, 37(9) |
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| 作者姓名： | 韩建勋 吴亚君 王斌 杨海荣 陈颖 |
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| 作者单位： | 中国检验检疫科学研究院,北京,100123 |
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| 基金项目： | 转基因重大专项课题(2008ZX08012-001); 国家863计划(2011AA1008047) |
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| 摘 要： | 以大豆卵磷脂为研究对象,比较了CTAB法、磁珠试剂盒法以及硅胶膜试剂盒法等3种DNA提取纯化方法。通过大豆内源基因Lectin实时荧光PCR检测,发现CTAB法提取的大豆卵磷脂DNA质量优于其它两种方法,并以CTAB法为基础进行条件优化,进一步提高DNA纯度。采用该方法对6个不同品牌的市售大豆卵磷脂产品进行转基因成分(CAMV35S启动子及NOS终止子)检测,结果证实均不含转基因成分。
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| 关 键 词： | 大豆卵磷脂 DNA提取 实时荧光PCR |
Study and Application of DNA Extraction Methods for Soybean Lecithin |
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| Han Jian-xun,Wu Ya-jun,Wang Bin,Yang Hai-rong,ChenYing. Study and Application of DNA Extraction Methods for Soybean Lecithin[J]. Food and Fermentation Industries, 2011, 37(9) |
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| Authors: | Han Jian-xun Wu Ya-jun Wang Bin Yang Hai-rong ChenYing |
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| Affiliation: | Han Jian-xun,Wu Ya-jun,Wang Bin,Yang Hai-rong,ChenYing(Institute of Food Safety,Chinese Academy of Inspection and Quarantine,Beijing 100123,China) |
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| Abstract: | The difficulty of detecting genetically modified products lies in DNA extraction from deep-processing products.In this study,DNA from soybean lecithin which was chose as the research object,was extracted and purified by CTAB method,bead kit and NucleoSpin Food kit.The purified DNA was detected by real-time PCR using soybean lectin gene which was one of soybean endogenous genes.The detecting result showed that CTAB method was suitable for DNA extraction of soybean lecithin.By optimizing the conditions of CTA... |
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| Keywords: | soybean lecithin DNA extraction real-time PCR |
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