| 白黄侧耳溶栓酶的分离纯化及特性分析 |
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| 引用本文： | 沈明花,金梅花,金成俊.白黄侧耳溶栓酶的分离纯化及特性分析[J].食品与发酵工业,2011,37(9). |
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| 作者姓名： | 沈明花 金梅花 金成俊 |
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| 作者单位： | 1. 延边大学医学部分子生物学实验室,吉林延吉,133000
2. 韩国朝鲜大学校自然科学大学,韩国光州501—759 |
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| 基金项目： | 国家自然科学基金资助项目(No.30760150) |
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| 摘 要： | 采用离子交换层析、凝胶过滤层析及快速蛋白液相色谱等蛋白质分离纯化技术,从白黄侧耳菌丝体中纯化一种溶栓酶,并进行酶学性质研究。实验结果表明,其分子质量约为18 ku,该酶的最适pH和最适温度为pH 7.0,40℃,并对糜蛋白酶底物S-2586最敏感。Co2+和Mg2+激活该酶活性,而Cu2+、EDTA对酶活有抑制作用。该酶不仅可直接水解纤维蛋白,还可以水解纤维蛋白原。
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| 关 键 词： | 白黄侧耳 溶栓酶 纯化 溶栓活性 |
Pruification of Fibrinolytic Enzyme from Cultured Mycelia of Pleurotus cornucopiae |
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| Shen Ming-hua,Jin Mei-hua,Kim Sung-jun.Pruification of Fibrinolytic Enzyme from Cultured Mycelia of Pleurotus cornucopiae[J].Food and Fermentation Industries,2011,37(9). |
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| Authors: | Shen Ming-hua Jin Mei-hua Kim Sung-jun |
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| Affiliation: | Shen Ming-hua1,Jin Mei-hua1,Kim Sung-jun2 1(Medical College Yanbian University,Yanji 133000,China) 2(Department of Natural Science,Chosun University,Gwang-Ju 501-759,Korea) |
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| Abstract: | A Fibrinolytic enzyme was purified from the mycelia of Pleurotus cornucopiae by ion exchange chromatography followed by gel filtration and fast protein liquid chromatography.Study the factors affected on the fibrinolytic activity of fibrinolytic enzyme from the mycelia of Pleurotus cornucopiae,and examine the fibrinolytic specialty on fibrin and fibrinogen and amidolytic activity.The apparent molecular mass of purified enzyme was estimated to be 18 ku.Its optimal pH and temperature value were 7.0 and 40℃,respectively,and it was found to exhibit a higher specificity for the substrate S-2586 for chymotrypsin.Enzyme activity was enhanced by Co2+ and Mg2+,but inhibited by the addition of Cu2+ and EDTA.The mycelia of Pleurotus cornucopiae may thus represent a potential source of new therapeutic agents to treat thrombosis. |
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| Keywords: | Pleurotus cornucopiae fibrinolytic enzyme purification fibrinolytic activity |
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