北京市食源性非伤寒沙门菌的分子分型和耐药性研究

目的了解北京市食源性非伤寒沙门菌的分子特征及耐药情况。方法对2004—2010年北京市食源性致病菌监测网收集的100株沙门菌进行脉冲场凝胶电泳(PFGE)分型和抗生素敏感性检测。结果 100株非伤寒沙门菌通过PFGE分型分为62个不同的带型,每个带型包含1~11株菌。抗生素敏感性结果显示,100株菌中有55株菌表现为对至少1种抗生素耐药,其中多重耐药菌株15株。菌株对各抗生素的耐药率为萘啶酸40%、四环素30%、氯霉素15%、庆大霉素10%、甲氧苄啶/磺胺甲恶唑10%、环丙沙星9%、头孢西丁1%、头孢噻肟0%。结论沙门菌PFGE带型和耐药谱均与血清型存在很高的一致性。提示北京市食源性非伤寒沙门菌的耐药情况比较严重,开展对该菌分子分型与耐药特征分析的联合监测意义重大。

Molecular subtyping and antibiotic resistance of nontyphoidal Salmonella isolated from food in Beijing

To investigate the molecular characteristics and antibiotic resistance of nontyphoidal Salmonella isolated from food in Beijing.Methods A total of 100 strains were isolated from foodborne pathogenic bacteria monitoring network in Beijing from 2004 to 2010, and were analyzed by PFGE and antimicrobial susceptibility test. Results The isolates were divided into 62 PFGE pattern, and each contains 1-11 strains. The result of antimicrobial susceptibility test showed that 55 strains were resistant to at least one antibiotic, including 15 multidrug resistant strains. The resistance rate to the eight antibiotics were nalidixic acid (40%), tetracycline (30%), chloramphenicol (15%), gentamicin (10%), trimethoprim/sulfamethoxazole (10%), ciprofloxacin (9%), cefoxitin (1%), and cefotaxime (0%).Conclusion PFGE profiles, antibiotic resistance patterns and serotypes of Salmonella showed high consistency. The antibiotic resistance of foodborne nontyphoidal Salmonella in Beijing was serious, and enhancing the joint monitoring of molecular subtyping and antibiotic resistance has significant importance.