Molecular characterization of Italian rice cultivars |
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Authors: | Alessandra Cirillo Stefania Del Gaudio Giovanni Di Bernardo Umberto Galderisi Antonino Cascino Marilena Cipollaro |
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Affiliation: | (1) Section of Biotechnology and Molecular Biology Antonino Cascino, Department of Experimental Medicine, Second University of Naples, Via Costantinopoli 16, 80138 Naples, Italy |
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Abstract: | Random amplified polymorphic DNA (RAPD) is an efficient tool to detect DNA polymorphisms and to distinguish different clones and cultivars. We have applied the RAPD technique to distinguish, starting from a single rice seed, among some Italian rice cultivars from market and from National Institute for research on food and nutrition, Italy. We also estimated the genomic similarity among the rice cultivars object of this study taking advantage of the amplification patterns. Despite the low genomic variability among Italian rice cultivars, RAPD technique allowed us to distinguish among them. This indicates that RAPD can be used routinely to verify the identity of Italian rice cultivars in order to prevent fraudulent commercial activity. |
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Keywords: | Genetic similarity Oryza sativa RAPD Cultivar DNA polymorphism Food traceability |
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