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Molecular characterization of Italian rice cultivars
Authors:Alessandra Cirillo  Stefania Del Gaudio  Giovanni Di Bernardo  Umberto Galderisi  Antonino Cascino  Marilena Cipollaro
Affiliation:(1) Section of Biotechnology and Molecular Biology Antonino Cascino, Department of Experimental Medicine, Second University of Naples, Via Costantinopoli 16, 80138 Naples, Italy
Abstract:Random amplified polymorphic DNA (RAPD) is an efficient tool to detect DNA polymorphisms and to distinguish different clones and cultivars. We have applied the RAPD technique to distinguish, starting from a single rice seed, among some Italian rice cultivars from market and from National Institute for research on food and nutrition, Italy. We also estimated the genomic similarity among the rice cultivars object of this study taking advantage of the amplification patterns. Despite the low genomic variability among Italian rice cultivars, RAPD technique allowed us to distinguish among them. This indicates that RAPD can be used routinely to verify the identity of Italian rice cultivars in order to prevent fraudulent commercial activity.
Keywords:Genetic similarity   Oryza sativa   RAPD  Cultivar  DNA polymorphism  Food traceability
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