| 超滤分离蛋白质过程快速优化新技术及其应用 |
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| 引用本文： | 万印华,陈向荣,马光辉,苏志国,崔占峰.超滤分离蛋白质过程快速优化新技术及其应用[J].膜科学与技术,2011,31(3):228-233. |
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| 作者姓名： | 万印华 陈向荣 马光辉 苏志国 崔占峰 |
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| 作者单位： | 1. 中国科学院过程工程研究所生物化T国家重点实验室,北京,100080
2. 英国牛津大学工程系,牛津OX1 3PJ |
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| 摘 要： | 介绍了蛋白质超滤分离快速优化新技术、新方法,包括脉冲进样技术,载体相超滤技术、参数连续变化超滤技术以及在这些技术基础上建立的超滤分离快速优化新方法,克服了常规蛋白质超滤分离过程优化中存在的实验工作量大、蛋白质消耗多、费时以及费用高等缺点.利用该方法对以溶菌酶和卵清蛋白为模型蛋白质的混合体系进行了研究,筛选出合适的超滤膜...
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| 关 键 词： | 超滤 蛋白质 分离 优化 溶菌酶 |
A novel methodology for the rapid process optimization of protein ultrafiltration and its application |
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| WAN Yinhua,CHEN Xiangrong,MA Guanhui,SU Zhiguo,CUI Zhanfeng.A novel methodology for the rapid process optimization of protein ultrafiltration and its application[J].Membrane Science and Technology,2011,31(3):228-233. |
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| Authors: | WAN Yinhua CHEN Xiangrong MA Guanhui SU Zhiguo CUI Zhanfeng |
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| Affiliation: | WAN Yinhua1,CHEN Xiangrong1,MA Guanhui1,SU Zhiguo1,CUI Zhanfeng2(1.National Key Laboratory of Biochemical Engineering,Institute of Process Engineering,Chinese Academy of Sciences,Beijing 100080,China,2.Department of Engineering Science,University of Oxford,Oxford OX1 3PJ,UK) |
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| Abstract: | A novel methodology,which involves the application and combination of several newly developed techniques such as pulsed sample injection technique,carrier phase ultrafiltration and parameter scanning ultrafiltration,was introduced in this paper.With this methodology,the fractionation of the binary mixture of lysozyme and ovalbumin was performed with 30 kDa Biomax and YM membranes,respectively,and rapid process optimization realized.The experimental results showed that under the operating conditions examined... |
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| Keywords: | ultrafiltration protein separation optimization lysozyme |
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