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| 丝氨酸配基PVDF亲和膜脱除人血浆中内毒素的研究 | |
| 引用本文： | 徐堃,黄曼,吴礼光,徐秋萍,张林,陈欢林. 丝氨酸配基PVDF亲和膜脱除人血浆中内毒素的研究[J]. 膜科学与技术, 2011, 31(2) |
| 作者姓名： | 徐堃 黄曼 吴礼光 徐秋萍 张林 陈欢林 |
| 作者单位： | 1. 浙江大学化学工程与生物工程学系,教育部膜与水处理技术工程研究中心,杭州,310027 2. 浙江大学,医学院附属邵逸犬医院,杭州,310016 3. 浙江工商大学,环境科学与工程学院,杭州,310012 |
| 基金项目： | 浙江省科技厅科技计划项目(2007C33008); 中央高校基本科研业务费专项资金资助(教育部膜与水处理技术工程研究中心,浙江大学,KYJD09011) |
| 摘 要： | 以L-丝氨酸(Ser)为配基,1,6-己二胺为间隔臂,制备了一种新型PVDF中空纤维亲和膜用于人血浆中内毒素的脱除.通过FTIR、XPS对PVDF中空纤维膜,亲水化改性膜以及亲和膜的结构进行了表征;探讨了离子强度、pH值对亲和膜吸附内毒素能力的影响以及内毒素分子在亲和膜上的吸附动力学.实验考察了自制的亲和膜组件用于人血浆中内毒素的脱除效果.结果表明,亲和膜对内毒素的吸附能力为0.058 EU/cm2;自制的亲和膜组件对内毒素含量为0.42 EU/mL的人血浆样品清除率为43.8%;亲和膜对人血浆样品的其它生化指标的影响不大. |
| 关 键 词： | 内毒素 亲和膜 PVDF 丝氨酸 人血浆 |
Endotoxin removal from human plasma through PVDF affinity membrane containing Ser ligand |
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| XU Kun,HUANG Man,WU Liguang,XU Qiuping,ZHANG Lin,CHEN Huanlin. Endotoxin removal from human plasma through PVDF affinity membrane containing Ser ligand[J]. Membrane Science and Technology, 2011, 31(2) | |
| Authors: | XU Kun HUANG Man WU Liguang XU Qiuping ZHANG Lin CHEN Huanlin |
| Affiliation: | XU Kun1,HUANG Man3,WU Liguang2,XU Qiuping3,ZHANG Lin1,CHEN Huanlin1(1.Department of Chemical & Biochemical Engineering,Zhejiang University,Engineering Research Center of Membrane and Water Treatment Technology,MOE,Hangzhou 310027,China,2.College of Environ.Sci.& Eng.,Zhejiang Gongshang University,Hangzhou 310035,3.Sir Run Run Shaw Hospital,Hangzhou 310016,China) |
| Abstract: | A new affinity membrane,PVDF hollow fiber membrane with L-serine(Ser) ligand was prepared(with 1,6-hexanediamine as the space arm) for endotoxin removal from human plasma.FTIR and X-ray photoelectron spectroscopy(XPS) were used to analyse the surface of membranes.The effects of operation parameters,such as pH value and ionic strength,on the endotoxin adsorption capacity of the affinity membranes were investigated,so as to the affinity adsorption kinetics.Then,self-made affinity membrane module was used to r... |
| Keywords: | endotoxin affinity membrane PVDF Ser human plasma |
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