| 高效液相色谱法分析三氯蔗糖的含量 |
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| 引用本文： | 周维义,邓小娟. 高效液相色谱法分析三氯蔗糖的含量[J]. 食品科学, 2007, 28(7): 360-361 |
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| 作者姓名： | 周维义 邓小娟 |
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| 作者单位： | 天津大学分析中心； 天津大学分析中心 天津300072； 天津300072； |
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| 摘 要： | 目的:建立三氯蔗糖高效液相色谱测定方法。方法:采用DIKMAC18色谱柱(200mm×4.6mmi.d.5μm),流动相为甲醇-水(体积比为65:35),流速1.0ml/min,示差折光检测器,柱温40℃。结果:三氯蔗糖质量浓度在1.5～2000mg/L范围内与峰面积呈良好的线性关系,平均回收率为99.65%,相对标准偏差(RSD)为0.15%。结论:方法简便快速,操作稳定,经济环保,适用于三氯蔗糖的分析。
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| 关 键 词： | 高效液相色谱 三氯蔗糖 示差检测器 |
| 文章编号： | 1002-6630（2007）07-0360-02 |
| 修稿时间： | 2006-07-22 |
Determination of Sucralose by High Performance Liquid Chromatography |
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| ZHOU Wei-yi,DENG Xiao-juan. Determination of Sucralose by High Performance Liquid Chromatography[J]. Food Science, 2007, 28(7): 360-361 |
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| Authors: | ZHOU Wei-yi DENG Xiao-juan |
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| Affiliation: | Analytical Centre, Tianjin University, Tianjin 300072, China |
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| Abstract: | Objective: A high performance liquid chromatographic method was developed to determine sucralose. Method: The column of DIKMA C18 (250 mm × 4.6 mm i.d. 5 μm) and a mobile phase of methanol-water (65:35, V/V) at a flow rate of 1.0 ml/min and column temperature 40 ℃ with refractive index detector were adopeted. Results: The method is proved to be linear in the range of sucralose from 1.5～2000 mg/L. The recovery is 99.65%. The relative standard deviation (RSD) is 0.15%. Conclusion: The method is simple, fast and stable. It is suitable for quantitative analysis for sucralose production process. |
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| Keywords: | high performance liquid chromatography sucralose refractive index detector |
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