硫酸氨基葡萄糖对口服透明质酸缓解小鼠佐剂性关节炎的增效作用

目的：探讨硫酸氨基葡萄糖（glucosamine sulfate，GS）对口服透明质酸（hyaluronic acid，HA）缓解小鼠佐剂性关节炎的增效作用。方法：将50 只8 周龄ICR雄性小鼠随机分成5 组：正常组、模型组、HA组、GS组和HA＋GS组。除正常组以外，其余4 组小鼠左后足注射弗氏完全佐剂（freund’s complete adjuvant，FCA）建立佐剂性关节炎模型，然后进行4 周的HA和GS口服给药，并分别对小鼠体质量、足跖肿胀度进行测定，同时采用酶联免疫吸附法（enzyme-linked immunosorbent assay，ELISA）检测小鼠血清中白细胞介素-1（interleukin-1，IL-1）及肿瘤坏死因子-α（tumor necrosis factor-α，TNF-α）的含量。结果：口服HA和GS都能够减轻小鼠的足跖肿胀度，而同时口服HA和GS能起到更好的消肿效果，血清中IL-1及TNF-α的含量也显著降低。结论：给予小鼠口服HA＋GS，比单独服用HA、GS能更有效、更快地缓解小鼠的足跖肿胀症状，同时能够降低血清中IL-1及TNF-α含量，进而对小鼠佐剂性关节炎起到更好的治愈效果。

Synergism of Glucosamine Sulfate in Relieving Effect of Hyaluronic Acid by Oral Administration on Mice with Adjuvant Arthritis

Aim: To explore the synergism of glucosamine sulfate (GS) with hyaluronic acid (HA) by oral administration in
relieving adjuvant arthritis in mice with. Methods: Fifty 8-week-old ICR male mice were randomly divided into 5 groups
at random with an equal number of animals in each group: normal, model, HA, GS, and HA + GS groups. Except normal
group, the animal models with adjuvant arthritis from 4 other groups were established by injecting 0.1 mL of Freund’s
complete adjuvant (FCA) into the left rear paw, followed by oral administration of HA and/or GS for 4 weeks. The body
weights and swelling degree of mouse rear feet were evaluated respectively. The serum concentration of IL-1 and TNF-α
in mice were detected by enzymelinked immunosorbent assay (ELISA). Results: Swelling degree of mouse feet could be
inhibited by oral administration of HA or GS, and this inhibitory effect was more pronounced when both compounds were
administrated. In addition, an obvious decrease in IL-1 and TNF-α levels in serum was observed. Conclusion: In addition to
decreasing serum IL-1 and TNF-α levels, HA and GS could alleviate the swelling of mouse feet more effectively and rapidly
when used in combination than when used individually, so that exerting a better curative effect on mouse arthritis.