| 应用PCR-DGGE技术分析泡菜中乳酸菌的多样性 |
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| 引用本文： | 付琳琳,曹郁生,李海星,陈燕. 应用PCR-DGGE技术分析泡菜中乳酸菌的多样性[J]. 食品与发酵工业, 2005, 31(12): 103-105 |
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| 作者姓名： | 付琳琳 曹郁生 李海星 陈燕 |
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| 作者单位： | 南昌大学中德联合研究院,食品科学教育部重点实验室,南昌,330047 |
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| 摘 要： | 从泡菜液中抽提总DNA,扩增16S rDNA的V7-V8区,变性梯度凝胶电泳分离PCR片段,发现不同样品间的菌种差异显著。对片段直接测序和克隆测序,进行Blast分析,绘制进化树。结果表明,DGGE和克隆技术相结合是研究泡菜中乳酸菌多样性的可行方法。
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| 关 键 词： | 泡菜 多样性 PCR-DGGE 乳酸菌 |
| 收稿时间： | 2005-08-24 |
| 修稿时间： | 2005-10-20 |
The Diversity of Lactic Acid Bacteria from Traditional Chinese Pickles by PCR- DGGE |
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| Fu Linlin,Cao Yusheng,Li Haixing,Chen Yan. The Diversity of Lactic Acid Bacteria from Traditional Chinese Pickles by PCR- DGGE[J]. Food and Fermentation Industries, 2005, 31(12): 103-105 |
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| Authors: | Fu Linlin Cao Yusheng Li Haixing Chen Yan |
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| Affiliation: | Key Laboratory for Food Science of Ministry of Education, Jiangxi- OAI joint research institute, Nanchang University, Nanchang 330047,China |
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| Abstract: | Total DNA was extracted from the pickle juice.The V7-V8 regions of 16S rDNA were amplified by PCR and the PCR products were analyzed by denaturing gradient gel electrophoresis(DGGE).The DGGE profiles showed that different samples had different dominant lactic acid bacteria.DGGE bands were excised to sequence directly and cloning technique was used to confirm the sequences.All these sequences were blasted in Genbank followed by phylogenetic analysis.The results suggested that DGGE combined with cloning technique is a practicable protocol to research the diversity of complex communities from pickles microbes. |
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| Keywords: | pickles biodiversity PCR-DGGE lactic acid bacteria |
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