| 固相萃取-高效液相色谱法测定啤酒原辅料中黄曲霉毒素B_1 |
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| 引用本文： | 杜元正,蔡国林,高献礼,陆健.固相萃取-高效液相色谱法测定啤酒原辅料中黄曲霉毒素B_1[J].食品与发酵工业,2012,38(2):168-173. |
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| 作者姓名： | 杜元正 蔡国林 高献礼 陆健 |
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| 作者单位： | 1. 江南大学粮食发酵工艺及技术国家工程实验室,江苏无锡214122／江南大学工业生物技术教育部重点实验室,江苏无锡214122／江南大学生物工程学院,江苏无锡214122
2. 江南大学生物工程学院,江苏无锡,214122 |
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| 基金项目： | 江苏高校优势学科建设工程资助项目;教育部新世纪优秀人才支持计划，2010年江南大学引进人才科研启动项目 |
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| 摘 要： | 以啤酒酿造中主要原辅料(大麦麦芽、大麦、大米、小麦麦芽)为样品,比较了几种不同净化柱净化黄曲霉毒素B1(AFB1)的回收率,最终选择了硅胶-硅藻土-中性氧化铝混合柱作为净化柱,建立了一种检测啤酒原辅料中AFB1的反相高效液相色谱(RP-HPLC)方法。结果表明,AFB1的检测线性范围为0.5～50μg/kg,线性相关系数r为0.999 7。方法检出限为0.15μg/kg,加标回收率80.1%～109.2%,相对标准偏差(RSD)为0.23%～4.29%。该方法简便、准确、成本低廉,可用于啤酒原辅料中的AFB1的质量控制。
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| 关 键 词： | 啤酒 原辅料 黄曲霉毒素B1(AFB1) 固相萃取 高效液相色谱 |
Determination of Aflatoxin B1 in Raw Materials of Beer by SPE-HPLC |
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| Du Yuan-zheng,Cai Guo-lin,Gao Xian-li,Lu Jian.Determination of Aflatoxin B1 in Raw Materials of Beer by SPE-HPLC[J].Food and Fermentation Industries,2012,38(2):168-173. |
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| Authors: | Du Yuan-zheng Cai Guo-lin Gao Xian-li Lu Jian |
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| Affiliation: | 1,2,3 1(National Engineering Laboratory for Cereal Fermentation Technology,Jiangnan University,Wuxi 214122,China) 2(The Key Laboratory of Industrial Biotechnology,Ministry of Education,School of Biotechnology,Jiangnan University,Wuxi 214122,China) 3(School of Biotechnology,Jiangnan University,Wuxi 214122,China) |
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| Abstract: | By comparing the recoveries of different solid phase extraction columns for aflatoxin B1(AFB1) clean-up,the silicone-diatomite-neutral alumina mixed column was chosen,and the analytical method of Reversed Phase High Performance Liquid Chromatography(RP-HPLC) was developed for determination of aflatoxin B1 in raw materials of beer(barley malt,barley,rice and wheat malt).The results showed that the linear range of detection of AFB1 ranged from 0.5 μg/kg to 50 μg/kg.Standard curve correlation coefficient(r) was 0.9997.The detection limit was 0.15 μg/kg.The average recovery ranged from 80.1% to 109.2%,and the relative standard deviation(RSD) ranged from 0.23% to 4.29%.The method was simple,accurate and cost saving.It was also proposed to be used for the quality control of AFB1 in raw materials of beer. |
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| Keywords: | beer raw materials aflatoxin B1 solid phase extraction HPLC |
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