| 采用三硝基苯磺酸法测定乳基婴幼儿配方奶粉中的有效赖氨酸 |
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| 引用本文： | 李君艳,仇凯,钟其顶,于晓瑾,丁福强,熊正河,张泽生.采用三硝基苯磺酸法测定乳基婴幼儿配方奶粉中的有效赖氨酸[J].食品与发酵工业,2012,38(1):156-160. |
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| 作者姓名： | 李君艳 仇凯 钟其顶 于晓瑾 丁福强 熊正河 张泽生 |
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| 作者单位： | 1. 天津科技大学食品工程与生物技术学院,天津,300457
2. 中国食品发酵工业研究院,北京100027／全国食品发酵标准化中心,北京100027 |
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| 基金项目： | 质检公益性行业科研专项 |
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| 摘 要： | 采用三硝基苯磺酸法建立了乳基婴幼儿配方奶粉中有效赖氨酸的测定方法,研究了乙醚用量、水解时间以及三硝基苯磺酸的孵化时间对水解液吸光度的影响。方法线性范围为0.02～0.12 mg/mL,相关系数r2=0.999 3,检出限为3.72μg/mL,定量限为10.23μg/mL,样品重复性相对标准偏差RSD为1.55%～5.57%,回收率为86%～115%。
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| 关 键 词： | 婴幼儿配方奶粉 三硝基苯磺酸(TNBS) 有效赖氨酸 |
Determination of Available Lysine in Milk-Based Infant Formula Powders with 2,4,6-Trinitrobenzene Sulfonic Acid |
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| Li Jun-yan,Qiu Kai,Zhong Qi-ding,Yu Xiao-jin,Ding Fu-qiang,Xiong Zheng-he,Zhang Ze-sheng.Determination of Available Lysine in Milk-Based Infant Formula Powders with 2,4,6-Trinitrobenzene Sulfonic Acid[J].Food and Fermentation Industries,2012,38(1):156-160. |
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| Authors: | Li Jun-yan Qiu Kai Zhong Qi-ding Yu Xiao-jin Ding Fu-qiang Xiong Zheng-he Zhang Ze-sheng |
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| Affiliation: | 1(The Institute of Food Engineering & Biotechnology,Tianjin University of Science & Technology,Tianjin 300457,China) 2(China National Research Institute of Food & Fermentation Industries,Beijing 100027,China) 3(National Standardization Centre of Food & Fermentation Industry,Beijing 100027,China) |
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| Abstract: | A method of determination of available lysine in infant formula powder with 2,4,6-trinitrobenzene sulfonic acid(TNBS) was established.The ether usage,hydrolysis time and incubation time with TNBS were studied.Good linearity was obtained in the range of 0.02~0.12 mg/mL(r2=0.999 3);the determination limit was 3.72 μg/mL;and quantitative limit was 10.23 μg/mL and the sample spiked recoveries were in range of 86%~115% with the reproducibility RSD of 1.55%~5.57%. |
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| Keywords: | infant formula 2 4 6-trinitrobenzene sulfonic acid(TNBS) available lysine |
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