| 油菜蜂花粉水溶性蛋白的分离及其抗氧化活性 |
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| 引用本文： | 邓建军,杨海霞,曹炜,程妮,王毕妮,高慧. 油菜蜂花粉水溶性蛋白的分离及其抗氧化活性[J]. 食品与发酵工业, 2012, 38(2): 207-211 |
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| 作者姓名： | 邓建军 杨海霞 曹炜 程妮 王毕妮 高慧 |
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| 作者单位： | 1. 西北大学食品科学与工程系,陕西西安,710069
2. 西安交通大学医学院公共卫生系营养与食品卫生学学科,陕西西安,710061 |
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| 基金项目： | 西安市农业应用技术研究项目(NC10018，陕西省科学技术研究发展计划项目，西北大学校内基金 |
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| 摘 要： | 采用饱和硫酸铵法对油菜蜂花粉中的水溶性蛋白进行逐级分离提取,发现20%～40%浓度的硫酸铵对蜂花粉水溶性蛋白的分离效果最好,得率和回收率分别为711.54μg/g和29.91%;同时,对不同硫酸铵浓度(0%～20%、20%～40%、40%～60%、60%～80%和80%～100%)得到的水溶性蛋白的体外抗氧化活性进行测定,发现20%～40%浓度硫酸铵盐析得到的蛋白对DPPH和·OH自由基的清除能力最强;相同浓度下,各级水溶性蛋白对·OH自由基的清除能力均强于DPPH自由基。结合SDS-PAGE图谱分析表明,分子质量约为39.0 ku和约为58.0 ku的2种蛋白有可能在油菜蜂花粉水溶性蛋白的抗氧化反应中发挥着重要的作用。
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| 关 键 词： | 油菜蜂花粉 水溶性蛋白 分离 抗氧化活性 |
Study on Isolation and Antioxidant Activity Analysis of Water-soluble Protein from Rape Bee Pollen |
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| Deng Jian-jun,Yang Hai-xia,Cao Wei,Cheng Ni,Wang Bi-ni,Gao Hui. Study on Isolation and Antioxidant Activity Analysis of Water-soluble Protein from Rape Bee Pollen[J]. Food and Fermentation Industries, 2012, 38(2): 207-211 |
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| Authors: | Deng Jian-jun Yang Hai-xia Cao Wei Cheng Ni Wang Bi-ni Gao Hui |
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| Affiliation: | 1(Department of Food Science and Technology,Northwest University,Xi’an 710069,China) 2(Department of Nutriology and Food Sanitation,Medical School of Xi’an Jiaotong University,Xi’an 710061,China) |
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| Abstract: | Rape bee pollen water-soluble proteins(RBPWPs) were isolated by the process of the ammonium sulfate salting-out.The results showed that isolation with 20%～40 % concentration of ammonium sulfate is the best,the yield and recovery were 711.54 μg/g and 29.91 %,respectively.Also,antioxidative activities of RBPWPs with different concentration of ammonium sulfate precipitation(0%～20 %,20%～40 %,40%～60 %,60%～80 % and 80%～100 %) were determined in vitro.DPPH and ·OH radical scavenging assay showed that RBPWPs with 20%～40 % concentration of ammonium sulfate had the highest activity.Additionally,all RBPWPs scavenged ·OH radical activities were higher than DPPH.Combination with SDS-PAGE result,it indicated that RBPWPs with ～39.0 ku and ～58.0 ku molecular weight may play an important role in antioxidation reactions. |
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| Keywords: | rape bee pollen water-soluble protein isolation antioxidative activity |
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