| 磷酸盐对大肠杆菌发酵异亮氨酸的影响 |
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| 引用本文： | 孙家凯,吴晓娇,王晶,徐庆阳,谢希贤,陈宁.磷酸盐对大肠杆菌发酵异亮氨酸的影响[J].食品与发酵工业,2012,38(1):20-24. |
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| 作者姓名： | 孙家凯 吴晓娇 王晶 徐庆阳 谢希贤 陈宁 |
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| 作者单位： | 天津科技大学生物工程学院 工业发酵微生物教育部重点实验室,天津,300457 |
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| 基金项目： | 国家发改委高技术产业化专项项目:年产1 500吨支链氨基酸产业化示范工程 |
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| 摘 要： | 探究磷酸盐浓度对大肠杆菌E.coli TRFP发酵生产L-异亮氨酸的影响。利用30 L发酵罐进行分批补料发酵试验,考察磷酸盐浓度对E.coli TRFP发酵生产L-异亮氨酸过程中生物量、比生长速率、L-异亮氨酸产量、发酵液中乙酸及NH4+浓度变化。结果表明,发酵初期维持低浓度磷酸盐(2 g/L KH2PO4),后期补加2 g/LKH2PO4可有效减缓菌体衰退,最终使菌体生物量和异亮氨酸产量分别提高了24.5%和12.7%,且副产物乙酸含量明显降低。
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| 关 键 词： | 磷酸盐 大肠杆菌 比生长速率 异亮氨酸 |
Effect of Phosphate on the Process of Escherichia coli L-isoleucine Fermentation |
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| Sun Jia-kai,Wu Xiao-jiao,Wang Jing,Xu Qing-yang,Xie Xi-xian,Chen Ning.Effect of Phosphate on the Process of Escherichia coli L-isoleucine Fermentation[J].Food and Fermentation Industries,2012,38(1):20-24. |
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| Authors: | Sun Jia-kai Wu Xiao-jiao Wang Jing Xu Qing-yang Xie Xi-xian Chen Ning |
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| Affiliation: | (Key Laboratory of Industrial Fermentation Microbiology,Ministry of Education,College of Biological Engineering, Tianjin University of Science & Technology,Tianjin 300457,China) |
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| Abstract: | The effects of phosphate on the fermentation of L-isoleucine was studied with L-isoleucine producing strain E.coli TRFC.The effects of initial phosphate concentration in the fermentation process on biomass,the specific growth rate,yield of L-isoleucine,concentration of byproduct acetic acid and ammonium were studied by carrying out fed-batch fermentation of E.coli TRFC in 30-Liter fermentor.The results showed that maintaining the initial phosphate at the concentration of 2g/L and adding 2g/L phosphate into the broth in the late period of fermentation could effectively slow down bacterial recession.The biomass and production of L-isoleucine increased by 24.5% and 12.7%,while the amount of by-product acetic acid decreased significantly. |
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| Keywords: | phosphate Escherichia coli the specific growth rate L-isoleucine |
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