| 细菌胞壁破碎条件优化及高肽酶菌株的筛选 |
| |
| 引用本文： | 李永霞,秦礼康. 细菌胞壁破碎条件优化及高肽酶菌株的筛选[J]. 食品与发酵工业, 2012, 38(4): 89-93 |
| |
| 作者姓名： | 李永霞 秦礼康 |
| |
| 作者单位： | 贵州大学生命科学学院,贵州贵阳,550025 |
| |
| 基金项目： | 贵州省科学技术基金，贵州大学创新基金，贵州省科技重大专项 |
| |
| 摘 要： | 为构建细菌总肽酶活力的测定方法,筛选出肽酶高产的益酵细菌,采用单因素试验和L9(34)正交试验,以反应体系中游离氨基酸总量为肽酶活力指标,对细菌细胞破碎条件进行优化并测定供试菌株的总肽酶活力。结果表明,超声-酶解法破碎细菌细胞的最佳条件为:溶菌酶添加量200μL、超声功率400 W、时间4 min、时间间隔3 s。在此条件下测定153株革兰氏阳性杆菌的总肽酶活力,筛选出肽酶高产革兰氏阳性杆菌11株,分别是DF2-1、ZM1-1、ZM2-1、ZM3-1、DF3-1、DF3-2-1、ZMJ-5、ZMJ-2-6、ZMJ-1-1、DFM-4和WCF-2。
|
| 关 键 词： | 革兰氏阳性杆菌 破碎 总肽酶 发酵豆制品 |
Optimization of Gram- positive Bacillus Disruption and Screening of High Peptidase Strains |
| |
| Affiliation: | Li Yong-xia,Qing Li-kang (College of Life Science,Guizhou University,Guiyang 550025,China) |
| |
| Abstract: | To construct the method of determining global peptidase activity on gram-positive bacillus,a L9（34） orthogonal test based on single-factor experiments was designed to optimize the condition of gram-positive bacillus disruption.The content of total free amino is the main evaluation index.As results,the optimum conditions were additive volume of lysozyme 200 μL,ultrasonic power 400W,ultrasonic time 4 min,time interval 3s.11 G+ bacillus with high global peptidase were obtained from 153 strains through this method,numbered DF2-1,ZM1-1,ZM2-1,ZM3-1,DF3-1,DF3-2-1,ZMJ-5,ZMJ-2-6,ZMJ-1-1,DFM-4,WCF-2. |
| |
| Keywords: | G + bacillus disruption global peptidase fermented soybean product |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
