| 枇杷核黄酮类物质微波法提取及对羟自由基清除作用研究 |
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| 引用本文： | 焦士蓉,王玲.枇杷核黄酮类物质微波法提取及对羟自由基清除作用研究[J].食品科学,2006,27(9):84-87. |
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| 作者姓名： | 焦士蓉 王玲 |
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| 作者单位： | 1. 四川大学公共卫生学院,四川,成都,610041;西华大学生物工程学院,四川,成都,610041
2. 西华大学理化学院,四川,成都,610039 |
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| 摘 要： | 本文对微波法提取枇杷核中黄酮类物质进行了研究，通过单因素及L9（3^4）正交试验，得到最佳工艺条件：m（乙醇溶液）／m（枇杷核）=15，提取功率为296W，时间120s提取3次，50％的乙醇溶液。按此最佳方法总黄酮提取率为1．41％。微波法与传统溶剂提取法比较，具有提取时间短、提取率高等优点。同时研究了该提取物对羟自由基清除作用，黄酮类物质对羟自由基的清除作用呈线性相关，相关系数R^2为0．9865。
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| 关 键 词： | 枇杷核 微波提取 黄酮类化合物 羟自由基 清除 |
| 文章编号： | 1002-6630（2006）09-0084-04 |
| 收稿时间： | 2005-09-15 |
| 修稿时间： | 2005-09-15 |
Study on Flavonoids Extracted from Loquat Pit by MAE and Hydroxyr Radical Scavenging Effects |
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| JIAO Shi-rong,WANG Ling.Study on Flavonoids Extracted from Loquat Pit by MAE and Hydroxyr Radical Scavenging Effects[J].Food Science,2006,27(9):84-87. |
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| Authors: | JIAO Shi-rong WANG Ling |
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| Affiliation: | 1.School of Public Health,Sichuan University, Chengdu 610041, China; 2.School of Bioengineering, Xihua University, Chengdu 610039, China; 3.School of Physical and Chemical, Xihua University, Chengdu 610039, China |
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| Abstract: | The paper studied the extracting of flavonoids in loquat pit with microwave assisted extraction (MAE). The single factor and orthogonal design methods L9(34) were applied for the optimal technological conditions: m(ethanol)/m(loquat pit)=15, microwave power 296W and 3 times extracting with microwave for 120 seconds, and 50% ethanol. In these conditions, the yield of flavonoids is 1.41%. As compared with tradition ethanol extraction, the microwave extraction is a rapid method with higher yield. At the same time, a linear relationship exists between scavenging of hydroxyl radical and the extracting of flavonoids in loquat pit (R2=0.9865). |
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| Keywords: | loquat pit microwave assisted extraction (MAE) flavonoids hydroxyl radical scavenging |
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