| 高效液相色谱-电喷雾串联质谱法测定保健食品中水苏糖含量 |
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| 引用本文： | 苏娜,陈波,姚守拙.高效液相色谱-电喷雾串联质谱法测定保健食品中水苏糖含量[J].中国食品卫生杂志,2009,21(6). |
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| 作者姓名： | 苏娜 陈波 姚守拙 |
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| 作者单位： | 湖南师范大学化学研究所,湖南,长沙,410081 |
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| 摘 要： | 目的以质谱定性定量,测定保健品中水苏糖含量。方法采用高效液相色谱/电喷雾质谱联用法(HPLC-ESI/MS),色谱柱:瑞典KromasilNH2柱;流动相:甲醇+乙腈+0.1%甲酸氨水溶液=8+52+40(体积比);流速1.0ml/min。质谱采用电喷雾正离子模式(ESI+),对m/z689进行选择离子监测,扫描范围(m/z):620～700amu。结果水苏糖在80～120μg/ml范围内,峰面积和浓度呈良好的线性关系(r=0.9992);相对标准偏差(RSD)为0.73%;在3个添加水平下的回收率均在93.0%～105.8%。结论本方法快速、选择特异性高、分析时间短且无需衍生处理,抗干扰能力强,能准确快速测定水苏糖的含量。
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| 关 键 词： | 色谱法 高压液相 光谱法 质量 电喷雾电离 水苏糖 营养保健品 |
Determination of Stachyose in Health Food by High Performance Liquid Chromatography/Mass Spectrometry |
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| SU N,CHEN Bo,YAO Shou-zhuo.Determination of Stachyose in Health Food by High Performance Liquid Chromatography/Mass Spectrometry[J].Chinese Journal of Food Hygiene,2009,21(6). |
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| Authors: | SU N CHEN Bo YAO Shou-zhuo |
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| Affiliation: | Chemical Research Institute of Hunan Normal University;Hunan Changsha 410081;China |
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| Abstract: | Objective To develop a simple,rapid and accurate method to determine stachyose.Method A high performance liquid chromatography/electrospray ionization mass spectrometry(HPLC-ESI/MS)was used.The analyte of samples were separated on a Kromasil NH_2 column with a ternary solvent mixture of methanol+acetonitrile +0.1% water solution of ammonia formate(8+52+40,v/v/v)as the mobile phase at a flow rate of 1.0 ml/min.Stachyose was identified and quantified by electrospray ionization mass spectrometry(ESI-MS),and th... |
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| Keywords: | Chromatography Liquid Spectrometry Mass Electrospray Ionization Stachyose Dietary Supplements |
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