高效液相色谱-二极管阵列法测定发酵面制品中合成着色剂

目的 建立高效液相色谱-二极管阵列法同时测定发酵面制品中的柠檬黄、胭脂红、日落黄、诱惑红和亮蓝5种合成色素的方法.方法 采用Kromasil C18色谱柱(250 mm×4.6 mm,10μm).以甲醇+乙腈(1+1)和乙酸铵溶液(0.01 mol/L,pH =4.0)为流动相,梯度洗脱,流速1.0 ml/min,用二极管阵列检测器记录各个色谱峰的紫外吸收光谱,色谱检测波长254 nm.结果 各色素在1.56～50.0μg/ml质量浓度范围内呈良好的线性关系,相关系数均为1.000 0.将该方法用于实际样品的检测,5种人工合成色素的加标回收率在98.9％ ～ 101.6％之间,相对标准偏差为0.11％～0.41％.结论 该方法操作简便、灵敏、结果可靠.

Simultaneous determination of synthetic colorants in fermented flour products by high-performance liquid chromatographic-diode array detector

Objective To develop a high performance liquid chromatographic-diode array detector for the simultaneous determination of Lemon Yellow,Carmine,Sunset Yellow,Allura Red and Bright Blue in fermented flour products.Methods The separation was performed on a Kromasil C18 column(250 mm×4.6 mm,10 μm) with methanol/acetonitrile(1∶ 1,V/V) and ammonium acetate(0.01 mol/L,pH=4.0)as the mobile phase at the flow rate of 1.0 ml/min.The detection wavelength was 254 nm and the peaks of UV spectra were captured by a photodiode array detector(DAD).Results The method showed a good linearity in the range of 1.56-50.0 μg/ml(r=1.000 0).The average recoveries of colorants ranged from 98.9% to 101.6%,with RSDs of 0.11%-0.41%.Conclusion The method is simple,accurate,sensitive and reliable.